Evaluation of three novel soil bacterial strains for efficient biodegradation of persistent boscalid fungicide: Kinetics and identification of microbial biodegradation intermediates. (1st January 2023)

• Record Type: Journal Article
• Authors: Bhatt, Devesh; Srivastava, Anjana; Srivastava, P.C.; Sharma, Anita
• Is Part Of: Environmental pollution. Volume 316(2023)part 1
• Publication Date: 2023-01-01
• Subjects: Boscalid -- Bacteria -- Biodegradation -- Kinetics -- Metabolites; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.120484
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Boscalid, a new fungicide of anilide group, is intended to prevent and treat grey mould ( Botrytis cinerea ), primarily in vines and other fruit plants. In many regions, its long half-life in soil and water poses a serious environmental threat. Boscalid is reported to be toxic to a variety of aquatic organisms. One of the best ways to lessen the amount of boscalid that gets into surface and ground waters is to reduce its concentration in soil. Soil microbes are crucial for the degradation of organic pollutants including pesticides. The present study reports the assessment of three novel soil bacterial strains isolated from pesticide-contaminated soil of Crop research centre, Pantnagar, Uttarakhand, India, which possess boscalid degradation ability. Two of these bacterial isolates could degrade boscalid up to 85–95% within 36 h of incubation period under shaking conditions in the minimal medium. The growth pattern of degrading bacterial isolates was monitored by recording the optical density (OD) of bacterial suspension using an ultra violet (UV)-visible spectrophotometer, whereas the concentration of primary boscalid was recorded by High-Performance Liquid Chromatography (HPLC-UV). A linear relationship was observed between the bacterial growth and the decrease in the residual concentration of boscalid. The concentration of boscalid during incubation with different bacterial strains could be best predicted by a second-order polynomial relationship with time and OD of the suspension as independent variables. Three degradation intermediates of boscalid namely, N-(1, 1′-biphenyl-2-yl)pyridine-3-carboxamide (C18 H14 N2 O, N-{[1, 1′-biphenyl]-2-yl}-2-chloropyridine-3-carboxamide (C18 H13 N2 OCl), and N-{[4′-chloro-1, 1′-biphenyl]-2-yl}-2-chloropyridine ({C17 H11 NCl2 }OH) were identified by the liquid chromatography-mass spectrometry (LC-MS) analysis of biodegraded samples. The biodegradation of boscalid through bacterial isolates seemed to be an economical and eco-friendly method for degrading a highly persistent boscalid fungicide. Graphical abstract: Image 1 Highlights: Boscalid degrading three bacterial strains isolated from contaminated soil. Bactreia could degrade up to 95% boscalid in 36 h of incubation time. Second order polynomial relationship best predicted boscalid concentration. Three metabolites were identified by LC-MS analysis.