1‐Bromopropane and 2‐bromopropane – Determination of 1‐bromopropane and 2‐bromopropane in urine by dynamic headspace GC/MS [Biomonitoring Methods, 2019]. Issue 1 (30th January 2019)

• Record Type: Journal Article
• Authors: Roßbach, B.; Rißler, E.; Budnik, L.T.; Finger, S.; Göen, Th.; Hartwig, A.
• Is Part Of: MAK collection for occupational health and safety. Volume 4:Issue 1(2019)
• Page Start: 317
• Page End: 336
• Publication Date: 2019-01-30
• Subjects: 1‐bromopropane -- 2‐bromopropane -- urine -- biomonitoring -- Analyses in Biological Materials -- headspace‐solid phase dynamic extraction -- HS‐SPDE -- gas chromatography mass spectrometry -- GC‐MS; Biological monitoring -- Periodicals; Industrial toxicology -- Periodicals; Hazardous substances -- Periodicals; 615.902
• DOI: 10.1002/3527600418.bi10694e2219
• Languages: English
• ISSNs: 2509-2383

Abstract

Abstract: The working group "Analyses in Biological Materials" of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method. The analytical method described herein permits the sensitive determination of both unmetabolised 1‐bromopropane and 2‐bromopropane in urine. Prior to the determination using GC‐MS in the Single‐Ion‐Mode (SIM), the analytes are extracted and enriched using Headspace Solid Phase Dynamic Extraction (SPDE). To this end, the urine samples are incubated at 50 °C and the analytes are extracted from the gas phase using SPDE. The headspace extract is then injected into the gas chomatograph for separation and mass spectrometric analysis. Calibration standards are prepared in urine and processed in the same way as the samples to be analysed. Deuterated benzene is used as the internal standard (IS).