Low‐coherent optical diffraction tomography by angle‐scanning illumination. Issue 5 (28th January 2019)

• Record Type: Journal Article
• Authors: Lee, KyeoReh; Shin, Seungwoo; Yaqoob, Zahid; So, Peter T. C.; Park, YongKeun
• Is Part Of: Journal of biophotonics. Volume 12:Issue 5(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-01-28
• Subjects: coherent noise -- low‐coherent -- optical diffraction tomography -- quantitative phase imaging; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201800289
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Temporally low‐coherent optical diffraction tomography (ODT) is proposed and demonstrated based on angle‐scanning Mach‐Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, the full‐field interference of incoherent light is successfully maintained during every angle‐scanning sequences. Further, current ODT reconstruction principles for temporally incoherent illuminations are thoroughly reviewed and developed. Several limitations of incoherent illumination are also discussed, such as the nondispersive assumption, optical sectioning capacity and illumination angle limitation. Using the proposed setup and reconstruction algorithms, low‐coherent ODT imaging of plastic microspheres, human red blood cells and rat pheochromocytoma cells is experimentally demonstrated. Abstract : Angle‐scanning low‐coherent optical diffraction tomography is introduced using the diffractive spatial modulation concept of incoherent light. The advantage and disadvantage of incoherent illumination is theoretically analyzed, and the optimal degree of incoherency is suggested as a conclusion. The suggested setup is experimentally demonstrated for various micro‐sized samples.