Periodontal regeneration using bone morphogenetic protein 2 incorporated biomimetic calcium phosphate in conjunction with barrier membrane: A pre‐clinical study in dogs. (7th October 2019)

• Record Type: Journal Article
• Authors: Wei, Lingfei; Teng, Fei; Deng, Liquan; Liu, Gangfeng; Luan, Mengyin; Jiang, Jie; Liu, Zhonghao; Liu, Yuelian
• Is Part Of: Journal of clinical periodontology. Volume 46:Number 12(2019)
• Page Start: 1254
• Page End: 1263
• Publication Date: 2019-10-07
• Subjects: bone morphogenetic protein 2 -- bone substitute -- periodontal tissue regeneration -- supra‐alveolar defect -- tissue engineering; Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.13195
• Languages: English
• ISSNs: 0303-6979

Abstract

Abstract: Aim: To evaluate the effect of bone morphogenetic protein 2 (BMP‐2) incorporated biomimetic calcium phosphate (BMP‐2/BioCaP) in conjunction with barrier membrane on periodontal regeneration in chronic periodontitis experimental model. Material and Methods: Chronic periodontitis experimental model with critical‐sized supra‐alveolar defects was created in 15 dogs' mandibles. After the initial periodontal therapy, the defects were randomly assigned to the following groups: (a) control; (b) barrier membrane; (c) deproteinized bovine bone mineral + barrier membrane; (d) BioCaP + barrier membrane and (e) BMP‐2/BioCaP + barrier membrane (6 quadrants with 18 teeth per group). Eight weeks later, clinical examinations, micro‐CT, and histomorphometric analyses were performed. Results: Clinical examinations, including plaque index, bleeding index, and probing depth, were similar for all groups. In contrast, the clinical attachment loss was significantly lower in defects grafted with BMP‐2/BioCaP and barrier membrane. The micro‐CT results showed that the height of mineralized tissue in defects grafted with BMP‐2/BioCaP and barrier membrane was significantly higher. For histometric analysis, the defects grafted with BMP‐2/BioCaP and barrier membrane exhibited significantly more connective tissue height, new cementum height, new bone height and area, as well as less down‐growth of junctional epithelium. Conclusion: BMP‐2/BioCaP could be a promising bone substitute for periodontal regeneration.