Matrine induces hepatocellular carcinoma apoptosis and represses EMT and stemness through microRNA-299-3p/PGAM1 axis. (25th November 2022)

• Record Type: Journal Article
• Authors: Wang, BaoLin; Wang, HuiHai; Zhao, Qin; Lu, Fei; Yan, ZhenZhuang; Zhou, Fang; Su, QingLun
• Is Part Of: Growth factors. Volume 40:Number 5/6(2022)
• Page Start: 200
• Page End: 211
• Publication Date: 2022-11-25
• Subjects: Matrine -- microRNA-299-3p -- hepatocellular carcinoma cells -- repression -- apoptosis; Growth factors -- Periodicals; Physiology -- Periodicals; 611
• DOI: 10.1080/08977194.2022.2113073
• Languages: English
• ISSNs: 0897-7194

Abstract

Abstract: This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA (miR)-299-3p / phosphoglycerate mutase 1 ( PGAM1 ) axis. The association between miR-299-3p expression with the prognosis of HCC patients was studied. miR-299-3p and PGAM1 sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between miR-299-3p and PGAM1 was identified. Matrine elevated miR-299-3p expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness in vitro . PGAM1 was a target of miR-299-3p . Repression of PGAM1 rescued the effects of miR-299-3p downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the miR-299-3p / PGAM1 axis.