Enhancing spectral quality in complex environmental matrices: Supporting suspect and non-target screening in zebra mussels with ion mobility. (December 2022)

• Record Type: Journal Article
• Authors: Menger, Frank; Celma, Alberto; Schymanski, Emma L.; Lai, Foon Yin; Bijlsma, Lubertus; Wiberg, Karin; Hernández, Félix; Sancho, Juan V.; Ahrens, Lutz
• Is Part Of: Environment international. Volume 170(2023)
• Publication Date: 2022-12
• Subjects: Polar organic chemical integrative sampler (POCIS) -- Mobility alignment -- Collision cross-section (CCS) -- Mass spectrum quality; Environmental protection -- Periodicals; Environmental health -- Periodicals; Environmental monitoring -- Periodicals; Environmental Monitoring -- Periodicals; Environnement -- Protection -- Périodiques; Hygiène du milieu -- Périodiques; Environnement -- Surveillance -- Périodiques; Environmental health; Environmental monitoring; Environmental protection; Periodicals; 333.705
• DOI: 10.1016/j.envint.2022.107585
• Languages: English
• ISSNs: 0160-4120

Abstract

Graphical abstract: Highlights: Complex environmental samples screened with IM-HRMS using MetFrag and PubChemLite. Mobility alignment improves spectrum quality and peak annotation. Thirty-two compounds tentatively identified using suspect and non-target screening. Predicted CCS values are valuable, automated implementation is not (yet) advised. Non-target screening in complex samples remains a major challenge. Abstract: Identification of bioaccumulating contaminants of emerging concern (CECs) via suspect and non-target screening remains a challenging task. In this study, ion mobility separation with high-resolution mass spectrometry (IM-HRMS) was used to investigate the effects of drift time (DT) alignment on spectrum quality and peak annotation for screening of CECs in complex sample matrices using data independent acquisition (DIA). Data treatment approaches (Binary Sample Comparison) and prioritisation strategies (Halogen Match, co-occurrence of features in biota and the water phase) were explored in a case study on zebra mussel ( Dreissena polymorpha ) in Lake Mälaren, Sweden's largest drinking water reservoir. DT alignment evidently improved the fragment spectrum quality by increasing the similarity score to reference spectra from on average (±standard deviation) 0.33 ± 0.31 to 0.64 ± 0.30 points, thus positively influencing structure elucidation efforts. Thirty-two features were tentatively identified at confidence level 3 or higher using MetFrag coupled with the new PubChemLite database, which included predicted collision cross-section values from CCSbase. The implementation of predicted mobility data was found to support compound annotation. This study illustrates a quantitative assessment of the benefits of IM-HRMS on spectral quality, which will enhance the performance of future screening studies of CECs in complex environmental matrices.