Comparative and Practical Aspects of Localization‐Based Super‐Resolution Imaging. Issue 1 (1st January 2013)
- Record Type:
- Journal Article
- Title:
- Comparative and Practical Aspects of Localization‐Based Super‐Resolution Imaging. Issue 1 (1st January 2013)
- Main Title:
- Comparative and Practical Aspects of Localization‐Based Super‐Resolution Imaging
- Authors:
- Laevsky, Gary S.
O'Connell, Christopher B. - Editors:
- Robinson, J. Paul
Darzynkiewicz, Zbigniew
Dobrucki, Jurek
Hyun, William C.
Nolan, John P.
Orfao, Alberto
Rabinovitch, Peter S. - Abstract:
- Abstract: Super‐resolution microscopy overcomes diffraction to generate images with superior resolution compared to conventional light microscopy. Localization‐based super‐resolution methods result in up to ten‐fold improvement in resolution by determining the positions of fluorescent molecules with sub‐pixel accuracy. This process critically depends on controlled emission at the level of individual fluorophores so that fluorescence is non‐overlapping, allowing for accurate centroid determination of diffraction‐limited spots by Gaussian fitting of the pixel intensities. The intrinsic photoswitching behavior of many fluorophores provides a convenient way to achieve emitter isolation. Here, we describe methods for label preparation and staining of cellular structures to obtain high‐quality images using localization super resolution. We also compare labeling strategies and dye characteristics relevant to all localization‐based techniques, such as STORM and PALM. Curr. Protoc. Cytom . 63:2.20.1‐2.20.11. © 2013 by John Wiley & Sons, Inc.
- Is Part Of:
- Current protocols in cytometry. Volume 63:Issue 1(2013)
- Journal:
- Current protocols in cytometry
- Issue:
- Volume 63:Issue 1(2013)
- Issue Display:
- Volume 63, Issue 1 (2013)
- Year:
- 2013
- Volume:
- 63
- Issue:
- 1
- Issue Sort Value:
- 2013-0063-0001-0000
- Page Start:
- 2.20.1
- Page End:
- 2.20.11
- Publication Date:
- 2013-01-01
- Subjects:
- super‐resolution microscopy -- STORM -- PALM -- tandem dyes
Cytology -- Laboratory manuals
Flow cytometry -- Laboratory manuals
Cell separation -- Laboratory manuals
Molecular biology -- Laboratory manuals
Flow Cytometry -- methods
Image Cytometry -- methods
Cell Separation -- methods
Cytological Techniques
Molecular Biology -- methods
Cell separation
Cytology
Flow cytometry
Molecular biology
Laboratory Manuals
Laboratory manuals
571.6 - Journal URLs:
- https://currentprotocols.onlinelibrary.wiley.com/journal/19349300 ↗
http://www3.interscience.wiley.com/cgi-bin/mrwhome/104554804/HOME ↗
http://rzblx1.uni-regensburg.de/ezeit/warpto.phtml?colors=7&jour_id=61791 ↗
http://onlinelibrary.wiley.com/ ↗ - DOI:
- 10.1002/0471142956.cy0220s63 ↗
- Languages:
- English
- ISSNs:
- 1934-9297
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 24551.xml