Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins. (1st February 2023)

• Record Type: Journal Article
• Authors: Wang, Xiaoqin; Li, Zhenhua; Li, Wenqing; Li, Cheng; Liu, Jing; Lu, Yu; Fan, Jiangbo; Ren, Haiyan; Huang, Linjuan; Wang, Zhongfu
• Is Part Of: Carbohydrate polymers. Volume 301(2023)Part A
• Publication Date: 2023-02-01
• Subjects: GDM gestational diabetes mellitus -- HILIC hydrophilic interaction chromatography -- MS mass spectrometry -- ESI electrospray ionization -- GP Girard's reagent P -- PMP 1-phenyl-3-methyl-5-pyrazolone -- PNGase F peptide:N glycosidase F; Human milk -- Gestational diabetes mellitus -- N/O-glycans -- HILIC-MS/MS; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120312
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Gestational diabetes mellitus (GDM) has negative effects on mothers and offspring, which may be related to the glycosylation level of milk proteins. Here, the human milk N/O -glycome of healthy and GDM individuals was analyzed by HILIC-MS/MS. A total of 56 putative N -glycans were detected, among which 12 N -glycans were significantly different between GDM and healthy milk. A total of 25 putative O -glycans were detected, and 11 of them varied greatly between GDM and healthy milk, especially H1N1S1 and H2N2S1. Overall, the relative content of N/O -glycans in GDM milk was significantly lower than that of healthy milk. In GDM milk, fucosylated N -glycans present higher proportion, whereas the proportion of sialylated O -glycans were lower. These findings would provide a foundation for in-depth study on the structure-activity relationship of milk N/O -glycans and are expected to drive the design of infant formula for newborns. Graphical abstract: Unlabelled Image