Genome-wide screening of altered m6A-tagged transcript profiles in the hippocampus after traumatic brain injury in mice. (May 2019)

• Record Type: Journal Article
• Authors: Wang, Yiqin; Mao, Jian; Wang, Xin; Lin, Yong; Hou, Guoqiang; Zhu, Jun; Xie, Baoshu
• Is Part Of: Epigenomics. Volume 11:Number 7(2019)
• Page Start: 805
• Page End: 819
• Publication Date: 2019-05
• Subjects: hippocampus -- N6-methyladenosine (m6A) -- traumatic brain injury (TBI); Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2019-0002
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: To systematically profile RNA m6A modification landscape after traumatic brain injury (TBI) in mice.Materials & methods: Expression of m6A-related genes was detected by quantitative real-time PCR (qPCR). Expression and location of METTL3, a key component of m6A methyltransferase complex, were determined by immunostaining. Genome-wide profiling of m6A-tagged transcripts was conducted by m6A-modified RNA immunoprecipitation sequencing (m6A-RIP-seq) and RNA sequencing (RNA-seq).Results: METTL3 was downregulated after TBI. In total, 922 m6A peaks were differentially expressed as determined by m6A-RIP-seq, with 370 upregulated and 552 downregulated. In addition, we identified differentially expressed hypomethylated and hypermethylated mRNA transcripts.Conclusion: Our data provided novel information regarding m6A modification changes in the early period of TBI, which might be promising therapy targets.