Submucosal microbiome of peri‐implant sites: A cross‐sectional study. (14th July 2021)

• Record Type: Journal Article
• Authors: Polymeri, Angeliki; van der Horst, Joyce; Buijs, Mark J.; Zaura, Egija; Wismeijer, Daniel; Crielaard, Wim; Loos, Bruno G.; Laine, Marja L.; Brandt, Bernd W.
• Is Part Of: Journal of clinical periodontology. Volume 48:Number 9(2021)
• Page Start: 1228
• Page End: 1239
• Publication Date: 2021-07-14
• Subjects: dental implant -- dysbiosis -- microbiome -- next‐generation sequencing; Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.13502
• Languages: English
• ISSNs: 0303-6979

Abstract

Abstract: Aim: To study the peri‐implant submucosal microbiome in relation to implant disease status, dentition status, smoking habit, gender, implant location, implant system, time of functional loading, probing pocket depth (PPD), and presence of bleeding on probing. Materials and Methods: Biofilm samples were collected from the deepest peri‐implant site of 41 patients with paper points, and analysed using 16S rRNA gene pyrosequencing. Results: We observed differences in microbial profiles by PPD, implant disease status, and dentition status. Microbiota in deep pockets included higher proportions of the genera Fusobacterium, Prevotella, and Anaeroglobus compared with shallow pockets that harboured more Rothia, Neisseria, Haemophilus, and Streptococcus . Peri‐implantitis (PI) sites were dominated by Fusobacterium and Treponema compared with healthy implants and peri‐implant mucositis, which were mostly colonized by Rothia and Streptococcus . Partially edentulous (PE) individuals presented more Fusobacterium, Prevotella, and Rothia, whereas fully edentulous individuals presented more Veillonella and Streptococcus . Conclusions: PPD, implant disease status, and dentition status may affect the submucosal ecology leading to variation in composition of the microbiome. Deep pockets, PI, and PE individuals were dominated by Gram‐negative anaerobic taxa.