Hydrophilic Gelatin and Hyaluronic Acid-Treated PLGA Scaffolds for Cartilage Tissue Engineering. Issue 1 (January 2013)

• Record Type: Journal Article
• Authors: Chang, Nai-Jen; Jhung, Yi-Ru; Yao, Chih-Kai; Yeh, Ming-Long
• Is Part Of: Journal of applied biomaterials & functional materials. Volume 11:Issue 1(2013)
• Page Start: 45
• Page End: 52
• Publication Date: 2013-01
• Subjects: Chondrocyte -- Extracellular matrix -- Surface modification -- Tissue engineering; Biomedical materials -- Periodicals; Bioengineering -- Periodicals; Biomechanics -- Periodicals; 610.28
• DOI: 10.5301/JABFM.2012.9253
• Languages: English
• ISSNs: 2280-8000

Abstract

Tissue engineering provides a new strategy for repairing damaged cartilage. Surface and mechanical properties of scaffolds play important roles in inducing cell growth. Aim: The aim of this study was to fabricate and characterize PLGA and gelatin/hyaluronic acid-treated PLGA (PLGA-GH) sponge scaffolds for articular cartilage tissue engineering. Methods: The PLGA-GH scaffolds were cross-linked with gelatin and hyaluronic acid. Primary chondrocytes isolated from porcine articular cartilages were used to assess cell compatibility. The characteristic PLGA-GH scaffold was higher in water uptake ratio and degradation rate within 42 days than the PLGA scaffold. Results: The mean compressive moduli of PLGA and PLGA-GH scaffolds were 1.72±0.50 MPa and 1.86±0.90 MPa, respectively. The cell attachment ratio, proliferation, and extracellular matrix secretion on PLGA-GH scaffolds are superior to those of PLGA scaffolds. Conclusions: In our study, PLGA-GH scaffolds exhibited improvements in cell biocompatibility, cell proliferation, extracellular matrix synthesis, and appropriate mechanical and structural properties for potential engineering cartilage applications.