In Vivo Micro‐Computerized Tomography Tracking of Human Periodontal Ligament Stem Cells Labeled with Gold Nanocomplexes. Issue 1 (9th November 2021)

• Record Type: Journal Article
• Authors: Zong, Chen; Bronckaers, Annelies; Vande Velde, Greetje; Willems, Guy; Cadenas de Llano‐Pérula, Maria
• Is Part Of: Advanced healthcare materials. Volume 11:Issue 1(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-11-09
• Subjects: cell tracking -- cellular imaging -- computed tomography -- gold nanoparticles -- nanomaterials -- periodontal ligament stem cells -- transplantation; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202101133
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Gold nanocomplexes have been proposed as contrast agents for computerized tomography (CT) and cell tracking, which is especially useful in stem cell therapy. However, their potential for long‐term in vivo cell detection is still unknown. This study proposes an optimized approach to labeling human periodontal ligament stem cells (hPDLSCs) with gold nanocomplexes to evaluate their detection with micro‐CT after transplantation at four different rat tissues. The gold nanocomplexes of 0.05 mg mL −1 do not affect cell viability nor osteogenic differentiation capacity, but render fluorescent and radiopaque hPDLSCs. Excellent linear correlation with the number of labeled cells is shown over a wide range ( r = 0.99, P < 0.01), with a detection limit of ≈1.2 × 10 3 cells/µL. In vivo, strong, and durable detection of transplanted labeled cells within 5 days at all investigated areas is seen by micro‐CT and immunohistochemical assay. This approach confirms the potential of gold nanocomplexes in longitudinal in vivo cell tracking, which may facilitate their application in CT image‐guided interventions commonly used in oromaxillofacial or systemic applications of stem cell therapy. Abstract : Gold nanoparticles (AuNPs) are synthesized as AuNP–poly‐l ‐lysine hydrobromide–rhodamine B isothiocyanate nanocomplexes and used as a nanoprobe to track transplanted oral stem cells in vivo for 5 days. The transplanted cells labeled with gold nanocomplexes are shown as hyperdense‐contrast areas by micro‐computerized tomography and verified to be alive in immunocompetent hosts by immunohistochemical assay.