3D Printed Bioelectronic Microwells. (20th April 2021)

• Record Type: Journal Article
• Authors: Katseli, Vassiliki; Angelopoulou, Michailia; Kokkinos, Christos
• Is Part Of: Advanced functional materials. Volume 31:Number 26(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-04-20
• Subjects: 3D‐printing -- biosensors -- electrochemistry -- microwells -- quantum dots; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202102459
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: The in‐house and on‐demand fabrication of electrochemical integrated biosensors is a great challenge, especially in the field of modern point‐of‐care diagnostics. 3D printing technology allows the production of specialized electronic devices adapted to the required conditions, and 3D printed thermoplastic electrodes have shown hopeful achievements mainly in enzymatic bioassays. This work describes a novel configuration of integrated all‐3D‐printed electrochemical microtitration wells (e‐wells) for direct quantum dot‐based (QDs) and enzymatic bioassays. The e‐wells enable the in situ development of complete bioassays, that is, from sample addition to biomarker detection, without the need for external equipment other than a micropipette and a detector. The bioanalytical capability of the 3D e‐wells is demonstrated through the voltammetric bioassay of C‐reactive protein employing biotinylated reporter antibody and streptavidin‐conjugated CdSe/ZnS QDs. In addition, in order to extend their scope to enzymatic biosensing, e‐wells are applied to the amperometric determination of hydrogen peroxide by‐products, demonstrating their universal applicability in electrochemical bioassays. Abstract : All‐3D‐printed bioelectronic microwells (e‐wells) are fabricated for the in situ development of quantum dot (QD)‐based and enzymatic bioassays. The bioanalytical capability of the 3D e‐wells is demonstrated through a QD‐based immunoassay of C‐reactive protein and via hydrogen peroxide determination. The e‐wells can be printed within a medical setting, covering the core request for specialized and low‐cost biosensors at the point‐of‐care.