IL‐10 induces MC3T3‐E1 cells differentiation towards osteoblastic fate in murine model. Issue 1 (21st November 2019)

• Record Type: Journal Article
• Authors: Xiong, Yuan; Yan, Chenchen; Chen, Lang; Endo, Yori; Sun, Yun; Zhou, Wu; Hu, Yiqiang; Hu, Liangcong; Chen, Dong; Xue, Hang; Mi, Bobin; Liu, Guohui
• Is Part Of: Journal of cellular and molecular medicine. Volume 24:Issue 1(2020)
• Page Start: 1076
• Page End: 1086
• Publication Date: 2019-11-21
• Subjects: Fracture -- IGF1R -- IL‐10 -- miRNA -- mRNA -- osteoblast; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.14832
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Interleukin‐10 (IL‐10) displays well‐documented anti‐inflammatory effects, but its effects on osteoblast differentiation have not been investigated. In this study, we found IL‐10 negatively regulates microRNA‐7025‐5p (miR‐7025‐5p), the down‐regulation of which enhances osteoblast differentiation. Furthermore, through luciferase reporter assays, we found evidence that insulin‐like growth factor 1 receptor (IGF1R) is a miR‐7025‐5p target gene that positively regulates osteoblast differentiation. In vivo studies indicated that the pre‐injection of IL‐10 leads to increased bone formation, while agomiR‐7025‐5p injection delays fracture healing. Taken together, these results indicate that IL‐10 induces osteoblast differentiation via regulation of the miR‐7025‐5p/IGF1R axis. IL‐10 therefore represents a promising therapeutic strategy to promote fracture healing.