MicroRNA‐1 promotes apoptosis of hepatocarcinoma cells by targeting apoptosis inhibitor‐5 (API‐5). Issue 1 (27th November 2014)

• Record Type: Journal Article
• Authors: Li, Dong; Liu, Yu; Li, Hua; Peng, Jing-Jing; Tan, Yan; Zou, Qiang; Song, Xiao-Feng; Du, Min; Yang, Zheng-Hui; Tan, Yong; Zhou, Jin-Jun; Xu, Tao; Fu, Zeng-Qiang; Feng, Jian-Qiong; Cheng, Peng; chen, Tao; Wei, Dong; Su, Xiao-Mei; Liu, Huan-Yi; Qi, Zhong-Chun; Tang, Li-Jun; Wang, Tao; Guo, Xin; Hu, Yong-He; Zhang, Tao
• Is Part Of: FEBS letters. Volume 589:Issue 1(2015)
• Page Start: 68
• Page End: 76
• Publication Date: 2014-11-27
• Subjects: HCC -- hepatocellular carcinoma -- API-5 -- apoptosis inhibitor 5 -- MET -- MET proto-oncogene -- HDAC4 -- histone deacetylase 4 -- FOXP1 -- forkhead box P1 -- CCND2 -- G1/S-specific cyclin-D2 -- CXCR4 -- C-X-C chemokine receptor type 4 -- SDF-1 -- stromal cell-derived factor 1 -- microRNA-1 -- Apoptosis inhibitor 5 -- Apoptosis -- Hepatocellular carcinoma -- Hepatoma cell; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1016/j.febslet.2014.11.025
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Although microRNA-1 ( miR-1 ) is a known liver cancer suppressor, the role of miR-1 in apoptosis of hepatoma cells has remained largely unknown. Our study shows that ectopic miR-1 overexpression induced apoptosis of liver hepatocellular carcinoma (HepG2) cells. Apoptosis inhibitor 5 ( API-5 ) was found to be a potential regulator of miR-1 induced apoptosis, using a bioinformatics approach. Furthermore, an inverse relationship between miR-1 and API-5 expression was observed in human liver cancer tissues and adjacent normal liver tissues. Negative regulation of API-5 expression by miR-1 was demonstrated to promote apoptosis of HepG2 cells. Our study provides a novel regulatory mechanism of miR-1 in the apoptosis of hepatoma cells. Abstract : miR-1 suppresses the malignant properties of HCC in vitro and in vivo. miR-1 and API-5 are inversely expressed in human primary HCC tissues. miR-1 negatively regulates API-5 expression via binding to its 3′UTR. miR-1 promotes apoptosis of hepatoma cells by directly targeting API-5 .