Distinct conformational and functional effects of two adjacent pathogenic mutations in cardiac troponin I at the interface with troponin T. Issue 1 (13th January 2015)

• Record Type: Journal Article
• Authors: Akhter, Shirin; Jin, J.-P.
• Is Part Of: FEBS open bio. Volume 5:Issue 1(2015)
• Page Start: 64
• Page End: 75
• Publication Date: 2015-01-13
• Subjects: Troponin -- Troponin I–Troponin T interface -- Troponin I mutation -- Cardiac muscle -- Protein conformation; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.fob.2015.01.001
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : The α‐helix in troponin I (TnI) at the interface with troponin T (TnT) is a highly conserved structure. A point mutation in this region, A116G, was found in human cardiac TnI in a case of cardiomyopathy. An adjacent dominantly negative mutation found in turkey cardiac TnI (R111C, equivalent to K117C in human and K118C in mouse) decreased diastolic function and blunted beta‐adrenergic response in transgenic mice. To investigate the functional importance of the TnI–TnT interface and pathological impact of the cardiac TnI mutations, we engineered K118C and A117G mutations in mouse cardiac TnI for functional studies. Despite their adjacent locations, A117G substitution results in faster mobility of cardiac TnI in SDS–PAGE whereas K118C decreases gel mobility, indicating significant and distinct changes in overall protein conformation. Consistently, monoclonal antibody epitope analysis demonstrated distinct local and remote conformational alterations in the two mutant proteins. Protein binding assays showed that K118C, but not A117G, decreased the relative binding affinity of cardiac TnI for TnT. K118C mutation decreased binding affinity for troponin C in a Ca 2+ ‐dependent manner, whereas A117G had a similar but less profound effect. Protein kinase A phosphorylation or truncation to remove the cardiac specific N‐terminal extension of cardiac TnI resulted in similar conformational changes in the region interfacing with TnT and minimized the functional impacts of the mutations. The data demonstrate potent conformational and functional impacts of the TnT‐interfacing helix in TnI and suggest a role of the N‐terminal extension of cardiac TnI in modulating TnI–TnT interface functions. Abstract : Mutations in the TnT‐interface of cardiac TnI cause cardiomyopathies. Mutations A117G and K118C resulted in distinct changes in protein conformation. K118C, but not A117G, decreased the binding affinity for TnT. K118C decreased binding affinity for TnC in a Ca 2+ ‐dependent manner – A117G had a similar but less profound effect. PKA treatment or N‐terminal truncation produces similar changes in cardiac TnI.