High glycosyltransferase 8 domain containing two protein levels contribute to poor prognosis in urothelial carcinoma. (9th August 2021)

• Record Type: Journal Article
• Authors: Chen, Tzu‐Ju; Chan, Ti‐Chun; Li, Chien‐Feng; Dilshan Sampath Dissanayaka, Dissanayakage; Kianpour, Maryam; He, Hong‐Lin; Huang, Steven K; Li, Wan‐Shan; Chen, Nai‐Yu; Shiue, Yow‐Ling
• Is Part Of: International journal of urology. Volume 28:Number 11(2021)
• Page Start: 1178
• Page End: 1187
• Publication Date: 2021-08-09
• Subjects: glycosyltransferase 8 domain containing protein 2 -- molecular pathology -- prognostic marker -- urothelial carcinoma; Urology -- Periodicals; Genitourinary organs -- Periodicals; Urologic Diseases -- Periodicals; 616.6005
• DOI: 10.1111/iju.14656
• Languages: English
• ISSNs: 0919-8172

Abstract

Abstract : Objective: To examine the expression levels of the glycosyltransferase 8 domain containing protein 2 and its clinical implications in urothelial carcinoma patients. Methods: Data mining, immunohistochemistry together with H ‐score calculation was carried out to evaluate the glycosyltransferase 8 domain containing protein 2 levels on tissue specimens from urothelial carcinoma patients, retrospectively. Correlations between glycosyltransferase 8 domain containing protein 2 H ‐score and imperative clinicopathological factors were measured. The indication of glycosyltransferase 8 domain containing protein 2 level on disease‐specific and metastasis‐free survivals were next analyzed. Results: In upper tract urothelial carcinomas ( n = 340) and bladder urothelial carcinomas ( n = 295), 170 (50%) and 148 (50%) patients, respectively, were identified to have high glycosyltransferase 8 domain containing protein 2 expression. The glycosyltransferase 8 domain containing protein 2 levels were correlated to several clinicopathological characteristics and patient survival. Upregulation of the glycosyltransferase 8 domain containing protein 2 was correlated to primary tumor ( P < 0.001), nodal metastasis ( P < 0.001), histological grade ( P < 0.001), vascular invasion ( P < 0.001), perineural invasion ( P < 0.05) and mitotic rate ( P < 0.001). High glycosyltransferase 8 domain containing protein 2 levels independently predicted poor disease‐specific survival ( P = 0.049) and metastasis‐free survival ( P = 0.008) in upper tract urothelial carcinoma and urinary bladder urothelial carcinoma, respectively. Gene Ontology enrichment analysis additionally showed that multiple biological processes were enriched including "ECM organization" (Gene Ontology:0030198), "extracellular structure organization" (Gene Ontology:0043062), "biological adhesion" (Gene Ontology:0022610), "cell adhesion" (Gene Ontology:0007155), "collagen fibril organization" (Gene Ontology:0030199) and "vasculature development" (Gene Ontology:0001944). Conclusions: The present findings suggest that upregulation of the glycosyltransferase 8 domain containing protein 2 is an independent and disadvantageous prognosticator in urothelial carcinoma. High glycosyltransferase 8 domain containing protein 2 level might play a crucial role in progression of urothelial carcinoma.