Detection of AFP by Electrochemical Immunosensor Based on Ag/Fe3O4/g‐C3N4. Issue 14 (8th June 2021)

• Record Type: Journal Article
• Authors: Zhang, Mengmeng; Cao, Kaihang; Mei, Lisha; Wang, Xiao; Liao, Xiaochen; Qiao, Xiuwen; Hong, Chenglin
• Is Part Of: ChemistrySelect. Volume 6:Issue 14(2021)
• Page Start: 3394
• Page End: 3398
• Publication Date: 2021-06-08
• Subjects: Alpha-fetoprotein (AFP) -- Magnetic iron oxide (Fe3O4) -- Immunosensor -- Graphite-like carbon nitride (g-C3N4); Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.202003896
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Herein, a novel electrochemical immunosensor was prepared to detect cancer alpha‐fetoprotein (AFP). An iron oxide/graphite‐phase carbon nitride/silver (Fe3 O4 −g‐C3 N4 −Ag) composite was used as the carrier. Metal‐free graphitic carbon nitrides (g‐C3 N4 ) have been recently recognized as high‐performance photocatalysts; however, their large‐scale applications are limited because of their poor electronic properties and low dispersion. Fe3 O4 particles were incorporated in the composite to improve the conductivity and dispersibility of g‐C3 N4 . Silver nanoparticles were prepared through UV irradiation and did not involve the use of reducing agents. A signal probe was prepared from Fe3 O4 −g‐C3 N4 −Ag and acetylene black. Cyclic voltammetry was conducted to detect AFP. Under optimized conditions, the proposed immunosensor exhibited excellent analytical abilities. The dynamic response range of the AFP concentration was 0.0001–80 ng ⋅ mL −1 . The detection limit was relatively low at 0.078 pg ⋅ mL −1 (S/N=3). The immunosensor also exhibited high specificity and good reproducibility. The results showed that the proposed immunosensor can be successfully used to detect AFP in human serum samples. Abstract : During the test, ultraviolet light is irradiated to reduce the silver ions to silver element in situ to generate electrical signals. The electrical signal decreases with the increase of the antigen concentration to detect the antigen concentration. The dynamic response range of the AFP concentration was 0.0001–80 ng ⋅ mL −1 . The detection limit was at 0.078 pg ⋅ mL −1 (S/N=3), thus the sensor has a wide detection range and a low detection limit.