Point‐of‐care detection of neutrophils in live skin microsamples using chemiluminescence. Issue 5 (23rd February 2020)

• Record Type: Journal Article
• Authors: Ibarra‐Silva, Esmeralda; Raff, Adam B.; Cardenas, Antonio; Franco, Walfre
• Is Part Of: Journal of biophotonics. Volume 13:Issue 5(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-02-23
• Subjects: chemiluminescence -- infection -- inflammation -- neutrophil -- skin; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201960170
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Many skin diseases are defined by the presence of neutrophils, which are among the first cells to respond to infection and inflammation. Currently, neutrophil identification in the skin is costly and slow. The objectives of the present work are to investigate the feasibility of detecting the presence of neutrophils in live skin microsamples using chemiluminescence and develop a device and procedures that will enable preclinical and clinical investigations. Our approach consists of collecting skin microsamples and exposing them to reagents that activate neutrophils and amplify the light emission produced by chemiluminescence. Experiments using live pig skin with and without inflammation show that it is feasible to detect the presence of neutrophils in the skin. The proposed method is minimally invasive, simple, fast, and does not require user specialization. The developed system is compact in size with a small footprint, which makes it portable and suitable for point‐of‐care diagnostics. Abstract : The presence of neutrophils defines many skin diseases. Neutrophils are among the first cells to respond to infection and inflammation. Currently, neutrophil identification in the skin is costly and slow. We developed a point‐of‐care detection system that utilizes skin micro‐biopsies to activate neutrophils and detect their light emission produced by chemiluminescence. In vivo, preclinical experiments show that it is feasible to detect the presence of neutrophils in the skin in minutes.