Label‐free stimulated Raman scattering imaging reveals silicone breast implant material in tissue. Issue 5 (23rd February 2020)

• Record Type: Journal Article
• Authors: van Haasterecht, Ludo; Zada, Liron; Schmidt, Robert W.; de Bakker, Erik; Barbé, Ellis; Leslie, Heather A.; Vethaak, A. Dick; Gibbs, Susan; de Boer, Johannes F.; Niessen, Frank B.; van Zuijlen, Paul P. M.; Groot, Marie Louise; Ariese, Freek
• Is Part Of: Journal of biophotonics. Volume 13:Issue 5(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-02-23
• Subjects: capsular contraction -- label‐free histology -- nonlinear optics -- pathology -- plastic surgery -- polymer detection -- silicone bleeding; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201960197
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant. SRS images coregistered with bright‐field images revealed the distribution and quantity of silicone material in the tissue. Fast and high‐resolution imaging of histology slides with molecular specificity using SRS provides an opportunity to investigate the role of silicone debris in the pathophysiology of implant‐linked diseases. Abstract : Leaking silicone material from breast implants has been hypothesized to be involved in implant‐related ailments. In tissue slides for conventional pathology, silicone material cannot be detected with certainty. We demonstrate an advanced label‐free imaging technique that proves the presence of silicone. The resulting image can be overlaid (in green) on a conventional histology slide, as shown here for breast implant capsule tissue. Scale bar: 100 μm.