Untargeted and targeted LC-MS and data processing workflow for the comprehensive analysis of oligopeptides from cyanobacteria. (January 2023)

• Record Type: Journal Article
• Authors: Varriale, Fabio; Tartaglione, Luciana; Zervou, Sevasti-Kiriaki; Miles, Christopher O.; Mazur-Marzec, Hanna; Triantis, Theodoros M.; Kaloudis, Triantafyllos; Hiskia, Anastasia; Dell'Aversano, Carmela
• Is Part Of: Chemosphere. Volume 311:Part 2(2023)
• Publication Date: 2023-01
• Subjects: Microcystins -- Cyanotoxins -- Cyanopeptides -- Untargeted LC-HRMS -- Targeted LC-MS/MS -- Data-dependent acquisition; AA amino acid -- Adda (2S, 3S, 4E, 6E, 8S, 9S)-3-amino-9-methoxy-2, 6, 8-trimethyl-10-phenyldeca-4, 6-dienoic acid -- Ahda 3-amino-2-hydroxydecanoic acid -- Ahp 3-amino-6-hydroxy-2-piperidone -- AP anabaenopeptin -- CID collision-induced dissociation -- ClAhda 3-amino-10-chloro-2-hydroxydecanoic acid -- Cl2Ahda 3-amino-10, 10-dichloro-2-hydroxydecanoic acid -- CPtp cyanopeptolin-type peptide -- CRM certified reference material -- DDA data-dependent acquisition -- Dha dehydroalanine -- Dhb dehydrobutyrine -- HCD higher-energy collisional dissociation -- Hty homotyrosine -- LC–HRMS liquid chromatography–high resolution mass spectrometry -- LC–MS liquid chromatography–mass spectrometry -- LC–MS/MS liquid chromatography–tandem mass spectrometry -- MC microcystin -- Mdha N-methyldehydroalanine -- MeAhda N-methyl-3-amino-2-hydroxydecanoic acid -- MeAsp methylaspartic acid -- MeIle N-methylisoleucine -- MeLeu N-methylleucine -- MePhe N-methylphenylalanine -- MeTyr N-methyltyrosine -- MG microginin -- MP micropeptin -- MRM multiple reaction monitoring -- Prhcys S-propylhomocysteine (propionine) -- Prhcys(O) S-propylhomocysteine sulfoxide -- QqQ triple quadrupole -- Rt retention time -- XIC extracted ion chromatogram; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.137012
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Cyanobacteria produce a plethora of structurally diverse bioactive secondary metabolites, including cyanotoxins which pose a serious threat to humans and other living organisms worldwide. Currently, a wide variety of mass spectrometry-based methods for determination of microcystins (MCs), the most commonly occurring and studied class of cyanotoxins, have been developed and employed for research and monitoring purposes. The scarcity of commercially available reference materials, together with the ever-growing range of mass spectrometers and analytical approaches, make the accuracy of quantitative analyses a critical point to be carefully investigated in view of a reliable risk evaluation. This study reports, a comparative investigation of the qualitative and quantitative MCs profile obtained using targeted and untargeted liquid chromatography–mass spectrometry approaches for the analyses of cyanobacterial biomass from Lake Kastoria, Greece. Comparison of the total MCs content measured by the two approaches showed good correlation, with variations in the range of 3.8–13.2%. In addition, the implementation of an analytical workflow on a hybrid linear ion trap/orbitrap mass spectrometer is described, based on combining data-dependent acquisition and a powerful database of cyanobacterial metabolites (CyanoMetDB) for the annotation of known and discovery of new cyanopeptides. This untargeted strategy proved highly effective for the identification of MCs, microginins, anabaenopeptins, and micropeptins. The systematic interpretation of the acquired fragmentation patterns allowed the elucidation of two new MC structural variants, MC-PrhcysR and MC-Prhcys(O)R, and proposal of structures for two new microginins, isomeric cyanostatin B and MG 821A, and three isomeric micropeptins at m / z 846.4715, 846.4711 and 846.4723. Graphical abstract: Image 1 Highlights: Comparison between untargeted LC-HRMS and targeted LC-MS/MS for microcystins quantitation. First finding of S-propylhomocysteine-containing microcystins. HRMS 2 -based structural elucidation of new microcystins. A novel data dependent acquisition-based workflow for cyanometabolites profiling. HRMS 2 -based structural insights of new microginins and cyanopeptoline-type peptides.