Profiling the molecular signature of satellite glial cells at the single cell level reveals high similarities between rodents and humans. Issue 12 (31st December 2022)

• Record Type: Journal Article
• Authors: Avraham, Oshri; Chamessian, Alexander; Feng, Rui; Yang, Lite; Halevi, Alexandra E.; Moore, Amy M.; Gereau, Robert W.; Cavalli, Valeria
• Is Part Of: Pain. Volume 163:Issue 12(2022)
• Page Start: 2348
• Page End: 2364
• Publication Date: 2022-12-31
• Subjects: Satellite glial cells -- DRG -- Pain -- Regeneration -- Human -- Mouse -- Rat -- Single-cell RNA-seq; Pain -- Periodicals; Douleur -- Périodiques; Anesthésie -- Périodiques; Pain; Electronic journals; Periodicals; Electronic journals; 616.0472
• DOI: 10.1097/j.pain.0000000000002628
• Languages: English
• ISSNs: 0304-3959

Abstract

Abstract : Supplemental Digital Content is Available in the Text. Key features of satellite glial cells, including their similarities with astrocytes and the enrichment of lipid metabolism pathways, are largely conserved between rodents and humans. Abstract: Peripheral sensory neurons located in dorsal root ganglia relay sensory information from the peripheral tissue to the brain. Satellite glial cells (SGCs) are unique glial cells that form an envelope completely surrounding each sensory neuron soma. This organization allows for close bidirectional communication between the neuron and its surrounding glial coat. Morphological and molecular changes in SGC have been observed in multiple pathological conditions such as inflammation, chemotherapy-induced neuropathy, viral infection, and nerve injuries. There is evidence that changes in SGC contribute to chronic pain by augmenting the neuronal activity in various rodent pain models. Satellite glial cells also play a critical role in axon regeneration. Whether findings made in rodent model systems are relevant to human physiology have not been investigated. Here, we present a detailed characterization of the transcriptional profile of SGC in mice, rats, and humans at the single cell level. Our findings suggest that key features of SGC in rodent models are conserved in humans. Our study provides the potential to leverage rodent SGC properties and identify potential targets in humans for the treatment of nerve injuries and alleviation of painful conditions.