Distinguishing colorectal adenoma from hyperplastic polyp by WNT2 expression. Issue 10 (3rd September 2021)

• Record Type: Journal Article
• Authors: Wang, Bangting; Wang, Xin; Tseng, Yujen; Huang, Meina; Luo, Feifei; Zhang, Jun; Liu, Jie
• Is Part Of: Journal of clinical laboratory analysis. Volume 35:Issue 10(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-03
• Subjects: bioinformatics -- colorectal adenoma -- extracellular matrix -- hyperplastic polyp -- Wnt signaling pathway -- WNT2; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23961
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Background: Colorectal adenoma (CRA) is a classical premalignant lesion, with high incidence and mainly coexisting with hyperplastic polyp (HPP). Hence, this study aimed to distinguish CRA from HPP by molecular expression profiling and advance the prevention of CRA and its malignance. Methods: CRA and paired HPP biopsies were collected by endoscopy. Through RNA‐sequencing (RNA‐seq), the differentially expressed genes (DEGs) were obtained. Functional enrichment analysis was performed based on the DEGs. The STRING database and Cytoscape were used to construct the protein‐protein interaction (PPI) network and perform module analysis. Hub genes were validated by real‐time quantitative PCR (RT‐qPCR) and immunohistochemistry. The ROC curve was drawn to establish the specificity of the hub genes. Results: 485 significant DEGs were identified including 133 up‐regulated and 352 down‐regulated. The top 10 up‐regulated genes were DLX5, MMP10, TAC1, ACAN, TAS2R38, WNT2, PHYHIPL, DKK4, DUSP27, and ABCA12 . The top 10 down‐regulated genes were SFRP2, CHRDL1, KBTBD12, RERGL, DPP10, CLCA4, GREM2, TMIGD1, FEV, and OTOP3 . Wnt signaling pathway and extracellular matrix (ECM) were up‐regulated in CRA. Three hub genes including WNT2, WNT5A, and SFRP1 were filtered out via Cytoscape. Further RT‐qPCR and immunohistochemistry confirmed that WNT2 was highly expressed in CRA. The area under the ROC curve (AUC) at 0.98 indicated the expression level of WNT2 as a candidate to differ CRA from HPP. Conclusion: Our study suggests Wnt signaling pathway and ECM are enriched in CRA, and WNT2 may be used as a novel biomarker for distinguishing CRA from HPP and preventing the malignance of CRA. Abstract : Collect colorectal hyperplastic polyp (HPP) and adenoma, and identify differentially expressed genes through comprehensive bioinformatic analysis. Then perform functional enrichment analysis and protein‐protein interaction, filter out significantly changed genes, and verify their expression at transcription and protein levels. WNT2 may be used as novel biomarker in distinguishing adenoma from HPP, and preventing the malignance of adenoma.