Antiallergic Effect of Anti–Siglec-F through Reduction of Eosinophilic Inflammation in Murine Allergic Rhinitis. Issue 3 (May 2013)

• Record Type: Journal Article
• Authors: Kim, Young Hyo; Park, Chang-Shin; Lim, Dae Hyun; Son, Byong Kwan; Kim, Jeong Hee; Ahn, Sung-Hye; Bochner, Bruce S.; Na, Kwangmin; Jang, Tae Young
• Is Part Of: American journal of rhinology & allergy. Volume 27:Issue 3(2013)
• Page Start: 187
• Page End: 191
• Publication Date: 2013-05
• Subjects: Nose -- Periodicals; Allergy -- Periodicals; 616.21005
• DOI: 10.2500/ajra.2013.27.3866
• Languages: English
• ISSNs: 1945-8924

Abstract

Background: Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods: Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n= 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results: Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion: Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.