Sequence variation and regulatory variation in acetylcholinesterase genes contribute to insecticide resistance in different populations of Leptinotarsa decemlineata. Issue 22 (1st November 2021)

• Record Type: Journal Article
• Authors: Margus, Aigi; Piiroinen, Saija; Lehmann, Philipp; Grapputo, Alessandro; Gilbert, Leona; Chen, Yolanda H.; Lindström, Leena
• Is Part Of: Ecology and evolution. Volume 11:Issue 22(2021)
• Page Start: 15995
• Page End: 16005
• Publication Date: 2021-11-01
• Subjects: carbamate -- gene expression -- insecticide resistance -- organophosphate -- target site mutation; Ecology -- Periodicals; Evolution -- Periodicals; 577.05
• DOI: 10.1002/ece3.8269
• Languages: English
• ISSNs: 2045-7758

Abstract

Abstract: Although insect herbivores are known to evolve resistance to insecticides through multiple genetic mechanisms, resistance in individual species has been assumed to follow the same mechanism. While both mutations in the target site insensitivity and increased amplification are known to contribute to insecticide resistance, little is known about the degree to which geographic populations of the same species differ at the target site in a response to insecticides. We tested structural (e.g., mutation profiles) and regulatory (e.g., the gene expression of Ldace1 and Ldace2, AChE activity) differences between two populations (Vermont, USA and Belchow, Poland) of the Colorado potato beetle, Leptinotarsa decemlineata in their resistance to two commonly used groups of insecticides, organophosphates, and carbamates. We established that Vermont beetles were more resistant to azinphos‐methyl and carbaryl insecticides than Belchow beetles, despite a similar frequency of resistance‐associated alleles (i.e., S291G) in the Ldace2 gene. However, the Vermont population had two additional amino acid replacements (G192S and F402Y) in the Ldace1 gene, which were absent in the Belchow population. Moreover, the Vermont population showed higher expression of Ldace1 and was less sensitive to AChE inhibition by azinphos‐methyl oxon than the Belchow population. Therefore, the two populations have evolved different genetic mechanisms to adapt to organophosphate and carbamate insecticides. Abstract : We tested structural and regulatory differences between two populations of the Colorado potato beetle in their resistance to two commonly used groups of insecticides. We show that despite the similar frequency of resistance‐associated mutations in the target site gene, the more resistant populations had additional mutations in another target site gene, higher expression of the target site genes and had less sensitive to enzyme to insecticide inhibition. Therefore, the two populations have evolved different genetic mechanisms to adapt to organophosphate and carbamate insecticides.