Elemental content and stoichiometry of SAR11 chemoheterotrophic marine bacteria. Issue 2 (9th February 2019)

• Record Type: Journal Article
• Authors: White, Angelicque E.; Giovannoni, Stephen J.; Zhao, Yanlin; Vergin, Kevin; Carlson, Craig A.
• Is Part Of: Limnology and oceanography letters. Volume 4:Issue 2(2019)
• Page Start: 44
• Page End: 51
• Publication Date: 2019-02-09
• Subjects: Limnology -- Periodicals; Oceanography -- Periodicals; 551.46
• DOI: 10.1002/lol2.10103
• Languages: English
• ISSNs: 2378-2242

Abstract

Abstract: We measured the carbon, nitrogen, and phosphorus content and production of cultured SAR11 cells in the genus Pelagibacter, from members of the 1a.1 and 1a.3 lineages, which are adapted to productive coastal waters and oligotrophic tropical/subtropical environments, respectively. The average growing SAR11 cell contained ~ 6.5 fg C, from which we calculated a global standing stock of 1.4 × 10 13 g C. Calculations that consider uncertainties in cell turnover rates and growth efficiencies indicate this stock could oxidize 6% to 37% of gross ocean primary production. We also found that SAR11 do not incorporate 3 H‐thymidine but do incorporate 3 H‐leucine. We estimate conversion factors of 0.74–1.51 kg C mol −1 leu, which are comparable to the low end of published leucine conversion factors for marine chemoheterotrophic bacterioplankton production. The molar ratio of elements C : N : P in growing cells was on average 25 : 6 : 1, significantly less than the mean (~ 50 : 10 : 1) for heterotrophic bacteria, indicating these strains are C and N poor relative to P.