Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical‐size bone defects. Issue 11 (19th December 2020)

• Record Type: Journal Article
• Authors: Matheus, Henrique R.; Ervolino, Edilson; Gusman, David Jonathan Rodrigues; Alves, Breno Edson Sendão; Fiorin, Luiz Guilherme; Pereira, Priscilla Aparecida; de Almeida, Juliano Milanezi
• Is Part Of: Journal of periodontology. Volume 92:Issue 11(2021)
• Page Start: 1646
• Page End: 1658
• Publication Date: 2020-12-19
• Subjects: animal experimentation -- biomarkers -- bone regeneration -- bone transplantation -- bone; Periodontics -- Periodicals; 617.632
• DOI: 10.1002/JPER.20-0613
• Languages: English
• ISSNs: 0022-3492

Abstract

Abstract: Background: This study is designed to evaluate the potential of different formulations of hyaluronic acid (HA) to improve new bone formation in critical‐size calvaria defect (CSD) when combined with a deproteinized bovine graft (DBG) material. Methods: Thirty male rats were used. A 5‐mm‐diameter CSD was created and three experimental groups (n = 10) were randomly assigned based on the treatments performed. Group DBG: CSD filled with a DBG; group DBG/LV: CSD filled by the combination of DBG and HA in a low‐viscosity crosslinking agent; group DBG/HV: CSD filled by the combination of DBG and HA in a high‐viscosity crosslinking agent. Animals were euthanized 30 days postoperatively. Histological, histometric (percentage of newly formed bone [PNFB], percentage of remaining graft particles, histochemical, and immunohistochemical (bone morphogenetic protein 2/4 [BMP2/4], osteocalcin [OCN], and tartrate‐resistant acid phosphatase [TRAP]) analyses were performed. Results: The highest PNFB was observed in DBG/HV when compared with the other groups ( P ≤0.05). DBG/LV and DBG/HV presented almost no inflammatory cells. In contrast, inflammation was observed in group DBG. Extensive resorption of graft particles was observed in group DBG, which was not present in DBG/LV and DBG/HV as confirmed by the larger size of the particles ( P ≤0.05). BMP2/4 and OCN immunolabeling were higher in DBG/HV when compared with group DBG ( P ≤0.05). Increased number of TRAP‐positive cells was observed in DBG/LV and DBG/HV ( P ≤0.05). Lower percentage of mature collagen fibers was observed in DBG/HV ( P ≤0.05). Conclusion: The combination of HA in a high‐viscosity crosslinking agent with DBG improves the bone repair process and increases the amount of newly formed bone towards CSDs in rat calvaria.