Back Cover: DNP‐Supported Solid‐State NMR Spectroscopy of Proteins Inside Mammalian Cells (Angew. Chem. Int. Ed. 37/2019). Issue 37 (25th July 2019)

• Record Type: Journal Article
• Authors: Narasimhan, Siddarth; Scherpe, Stephan; Lucini Paioni, Alessandra; van der Zwan, Johan; Folkers, Gert E.; Ovaa, Huib; Baldus, Marc
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 37(2019)
• Page Start: 13162
• Page End: 13162
• Publication Date: 2019-07-25
• Subjects: dynamic nuclear polarization -- in-cell NMR -- protein–protein interactions -- solid-state NMR -- ubiquitination; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201908849
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract : Dynamic nuclear polarization (DNP) enhanced solid‐state NMR (ssNMR) spectroscopy enables atomic‐level structural studies of isotope labeled proteins at physiologically relevant concentrations in mammalian cells, irrespective of the tumbling rate of the protein of interest, as reported by H. Ovaa, M. Baldus, and co‐workers in their Communication on page 12969 ff. In the absence of DNP, NMR sensitivity levels are insufficient to conduct such studies.