Redox‐State‐Mediated Regulation of Cytochrome c Release in Apoptosis Revealed by Surface‐Enhanced Raman Scattering on Nickel Substrates. Issue 46 (26th September 2019)

• Record Type: Journal Article
• Authors: Zhu, Jinyu; Jiang, Muwei; Ma, Hao; Zhang, Haijing; Cheng, Weina; Li, Junbo; Cai, Linjun; Han, Xiao Xia; Zhao, Bing
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 46(2019)
• Page Start: 16499
• Page End: 16503
• Publication Date: 2019-09-26
• Subjects: apoptosis -- cytochrome c -- nickel -- redox state -- SERS; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201909638
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The interaction of cytochrome c (Cyt c) with cardiolipin (CL) is believed to play an important role in the initial events of apoptosis. Herein, we investigate the structural changes of CL‐bound Fe 2+ Cyt c and the correlation with Cyt c release through surface‐enhanced Raman spectroscopy (SERS) on nickel substrates. The SERS results together with molecular dynamics simulation reveal that Fe 2+ Cyt c undergoes autoxidation and a relatively larger conformational alteration after binding with CL, inducing higher peroxidase activity of Cyt c and higher permeability of the CL membrane compared with those induced by the Fe 3+ Cyt c. The proapoptotic activity and SERS effect of the Ni nanostructures allow the in situ study of the redox‐state‐dependent Cyt c release from isolated mitochondria, which reveals for the first time that the ferrous state of Cyt c most likely plays a more important role in triggering apoptosis. Abstract : Bring a nickel, tap your feet : By using surface‐enhanced Raman spectroscopy on nickel substrates, it is shown that the redox state of cytochrome c (Cyt c ) regulates Cyt c release from mitochondria in apoptotic cells. The SERS data and MD simulations show that the ferrous state of Cyt c most likely plays a more important role in triggering apoptosis.