Chemical and Genetic Diversity of Ligularia kanaitzensis in the Hengduan Mountains Area. Chemical Relationship with L. subspicata. Issue 11 (20th October 2021)

• Record Type: Journal Article
• Authors: Hanai, Ryo; Okamoto, Yasuko; Hashida, Takuya; Shiojiri, Kosuzu; Ohsaki, Ayumi; Saito, Yoshinori; Tori, Motoo; Gong, Xun; Kuroda, Chiaki
• Is Part Of: Chemistry & biodiversity. Volume 18:Issue 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-20
• Subjects: terpenoids -- molecular evolution -- Ligularia kanaitzensis -- Ligularia subspicata -- Asteraceae; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Biodiversity -- Periodicals; 572
• DOI: 10.1002/cbdv.202100444
• Languages: English
• ISSNs: 1612-1872

Abstract

Abstract: Root chemicals and the sequences of the internal transcribed spacers (ITSs) were analyzed for 9 Ligularia kanaitzensis and 3 L. subspicata samples collected in northwestern Yunnan and southwestern Sichuan, China. Subspicatins A and C were isolated from two L. kanaitzensis samples. Introgression of genes responsible for these compounds from L. subspicata was suggested by their strong connection with L. subspicata / L. lamarum and the geographical proximity of the samples to L. subspicata . DNA analysis of a set of 27 L. kanaitzensis samples including those analyzed previously showed that they belong to two clades, designated A and B. Together with the presence/absence of furanoeremophilane, the 27 samples were sorted into three groups: clade A/furan, clade B/furan, and clade B/non‐furan. The ancestral plant presumably belonged to clade B/non‐furan, because furanoeremophilanes are biosynthesized from eremophilan‐8‐ones. 1β‐Angeloyloxyfukinone, a likely intermediate between fukinone and subspicatin C, was isolated for the first time. This finding allowed us to propose plausible biosynthetic pathways of subspicatins A and C. Abstract :