A novel dicyanisophorone-based colorimetric and ratiometric fluorescent probe for the detection of hypochlorite in the environment and living cells. (December 2022)

• Record Type: Journal Article
• Authors: Liu, Li; Yang, Yixian; Cui, Yuanyuan; Zhu, Weiju; Fang, Min; Li, Cun
• Is Part Of: Dyes and pigments. Volume 208(2023)
• Publication Date: 2022-12
• Subjects: Fluorescent probe -- ClO− -- Ratiometric -- Imaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2022.110879
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A novel dual emissive fluorescent probe SFQ was designed and synthesized by linking dicyanisophorone and thiophene aldehyde units to carbazole groups. The probe SFQ has high selectivity and sensitivity toward ClO − . Upon addition of ClO − to the solution of probe SFQ resulted in a noticeable color changed from orange to pink under natural light. Meanwhile, SFQ could make a ratiometric fluorescence response to ClO − at two different excitation wavelengths, with the change of color from orange-red fluorescence to cyan fluorescence under the 365 nm UV lamp. The detection limit was 1.4 × 10 −7 mol/L under ratiometric conditions. The study on the reaction mechanism showed that when SFQ reacted with ClO −, the cyano group disappeared and the cyclic ketone SFQ -O came into being. Furthermore, probe SFQ was successfully used to monitor ClO − in HepG2 cells and detect ClO − in actual environmental water samples. Graphical abstract: Image 1 Highlights: A novel probe SFQ for colorimetric and ratiometric identification of ClO − was prepared. SFQ can respond instantly to ClO − (<5 s) and has a large Stokes shift (145 nm). SFQ was successfully used to monitor ClO − in HepG2 cells and detect ClO − in actual environmental water samples.