Correlation between in vitro toxicity of pesticides and in vivo risk guidelines in support of complex operating site risk management: A meta-analysis. (December 2022)

• Record Type: Journal Article
• Authors: Alehashem, Maryam; Mamet, Steven; Hogan, Natacha; Hecker, Markus; Florou, Dimitra; Tsatsakis, Aristidis; Siciliano, Steven
• Is Part Of: Food and chemical toxicology. Volume 170(2022)
• Publication Date: 2022-12
• Subjects: Pesticides -- Human cells -- Toxicity -- IC50 -- In vivo risk guidelines; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2022.113502
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: In vitro cell systems can support hazard characterization and identify mechanisms involved in toxicity; however, using in vitro data for risk assessment still is challenging. As part of an effort to develop approaches for a complex operating site used for biocide packaging and distribution, we evaluated in vitro assays that could be used in a site management format. Across 66 studies, 108 pesticides were assessed on ten human-derived cell types at four endpoints. In vitro IC50s were compared to in vivo guidelines, NOEL/NOAELs, and ADIs using Spearman correlation and linear regression models. While human neuroblastoma cells (SH-SY5Y) were the most sensitive, HepG2 was the most used cell line in evaluating the toxicity of pesticides. Amongst the ten human cell lines, the IC50s derived from SH-SY5Y cells, using MTT-24 & 48 h (the most used assay) correlated (rho = 0.56–0.79; p < 0.05) with ADIs and NOEL/NOAELs. Although in vitro cell systems have some limitations, the correlation between in vitro data derived from SH-SY5Y cells and in vivo safety guidelines can provide site investigators with a tool to survey and prioritize areas and media of concern at complex operating sites impacted by pesticide mixtures. Highlights: The in vitro data derived from the SH-SY5Y cells were linked to the in vivo data. SH-SY5Y was the most sensitive cell line in assessing the toxicity of the pesticide. The IC50s from SH-SY5Y cells showed more consistency across the laboratories. SH-SY5Y can be proper cells for ranking the risk of unknown mixtures of pesticides.