Expression Characteristics Analysis of CesA/Csl genes in Flax During the Fast Growing Stage. Issue 14 (28th October 2022)

• Record Type: Journal Article
• Authors: Yuan, Hongmei; Guo, Wendong; Zhao, Lijuan; Yu, Ying; Chen, Si; Cheng, Lili; Kang, Qinghua; Song, Xixia; Wu, Jianzhong; Huang, Wengong; Liu, Yan; Tao, Lei; Wu, Guangwen
• Is Part Of: Journal of natural fibers. Volume 19:Issue 14(2022)
• Page Start: 7864
• Page End: 7877
• Publication Date: 2022-10-28
• Subjects: Flax -- CesA -- Csl -- expression pattern -- protein–protein interaction network -- secondary cell wall; 亚麻 -- CesA; Csl -- 表达模式 -- 蛋白质相互作用网络 -- 次生细胞壁; Textile research -- Periodicals; Textile research; Periodicals; 677.005
• DOI: 10.1080/15440478.2021.1958418
• Languages: English
• ISSNs: 1544-0478

Abstract

ABSTRACT: Expression characteristics of predicted CesA/Csl genes of fiber flax variety 'Diana' were investigated using RNA-seq data obtained during the fast-growing stage. First, flax stem anatomic analysis revealed differing developmental processes from bottom to top, with middle and lower stem fiber cells exhibiting significantly thickened cell walls. Moreover, stem cellulose/hemicellulose content exhibited positional biases, with a top-to-bottom increase in cellulose content and bottom-to-top increase in hemicellulose content observed. Most CesA/Csl genes were predominantly expressed in stems, roots, and less in leaves. The correlations between the expression levels of CesA/Csl genes and the contents of cellulose and hemicellulose were analyzed. The expression levels of LuCesA4, LuCesA8A and LuCesA8B genes showed significantly positive correlations with cellulose content ( P < 0.05), while expression levels of two LuCesA3, three LuCesA6, two LuCslA2, and four LuCslC genes revealed significantly positive correlations with hemicellulose content ( P < 0.05). CesA/Csl protein interaction network analysis revealed five transcription factors (TFs) that had high protein–protein interaction (PPI) scores with CesA/Csl proteins: LuNAC10 (Lus10015392), LuNAC12 (Lus10001664), LuNAM (Lus10017915), LuMyb42 (Lus10038913), LuMyb46 (Lus10027369). Therefore, these TFs may play critical regulatory roles in cellulose/hemicellulose synthesis. This study provides a theoretical foundation for investigating flax fiber cell wall synthesis and regulatory mechanisms.