Aqua-(2-formylbenzoato)triphenyltin(IV) induces cell cycle arrest and apoptosis in hypoxic triple negative breast cancer cells. (February 2023)

• Record Type: Journal Article
• Authors: Singh, Virendra; Rana, Nishant Kumar; Kashif, Mohammad; Manna, Partha Pratim; Basu Baul, Tushar S.; Koch, Biplob
• Is Part Of: Toxicology in vitro. Volume 86(2023)
• Publication Date: 2023-02
• Subjects: Hypoxia -- Apoptosis -- Cell cycle -- Doxorubicin -- Western blot -- Triple negative breast cancer cells; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2022.105484
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Hypoxia plays a vital role in tumor microenvironment by allowing development and maintenance of cancer cells thereby led to major hindrance for effective anticancer therapy and main reason for failure of most anticancer drugs. We herein investigated the therapeutic efficacy and molecular mechanism of action of aqua-(2-formylbenzoato) triphenyltin (IV) compound (OTC) in MDA-MB-231 cell line. Cobalt chloride induced hypoxic MDA-MB-231 cells treated with OTC were used to access cytotoxicity, ROS, cellular apoptosis, and cell cycle progression. Further, expression of HIF-1α and VEGF, as well as apoptotic proteins like p53, Bax, Bcl-2 and caspase 3 were assessed. The findings indicated that OTC is more effective towards CoCl2 induced hypoxic cells when compared to normoxic cells and the results are far superior to doxorubicin. Additionally, our study revealed that OTC facilitates more ROS production induced cell cycle arrest and promote apoptosis. Furthermore, OTC significantly down regulates the expression of Hif-1α, VEGF and Bcl-2 in hypoxic condition and elevates the level of p53, Bax, cytochrome-C and Caspase 3. Our in vitro studies demonstrated that OTC showed better efficacy than doxorubicin, corroborating that OTC could be a promising compound for hypoxic cancer that also display multi drug resistant. Highlights: Newly synthesized OTC suppresses the growth of hypoxia induced breast cancer cells. OTC induces cell cycle delay and promote apoptosis in p53 dependent manner. In-silico analysis confirms strong interaction with DNA and p53 and Hif-1α protein.