Exosome proteomics study of the effects of traditional cigarettes and electronic cigarettes on human bronchial epithelial cells. (February 2023)

• Record Type: Journal Article
• Authors: Wang, Weiling; Zeng, Rong; Liu, Min; Chen, Mulan; Wei, Shiqin; Li, Bowen; Yu, Suhong
• Is Part Of: Toxicology in vitro. Volume 86(2023)
• Publication Date: 2023-02
• Subjects: Electronic cigarette(e-Cig) -- Traditional cigarette(t-Cig) -- 16HBE cells -- Exosome proteomics -- Cytotoxicity; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2022.105516
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Electronic cigarette (e-Cig) has been promoted as a safer alternative to traditional cigarette (t-Cig) recently. However, there are limited scientific data on the potential health effects of e-Cig use. In this study, we evaluated the cytotoxicities of e-Cig and t-Cig condensate solutions (e-CigCS and t-CigCS) on human bronchial epithelial cells (16HBE cells) in vitro, and employed the exosome proteomic technique to systematically assess the effects of e-CigCS and t-CigCS on 16HBE cells. Cytotoxicity assay showed 16HBE cells were more sensitive to t-CigCS than e-CigCS. Proteomic analysis demonstrated that there are 431 differential expressed exosomal proteins (DEEPs) in test groups compared to the control air group ( P -value<0.05) and t-CigCS has a greater influence than e-CigCS on exosomal protein expression. Bioinformatic analysis showed the DEEPs from the t-Cig group were significantly enriched in pathways in cancer while tobacco-flavored e-Cig (e-Cig t ) and menthol-flavored e-Cig (e-Cig m ) groups were not. Further validations of some DEEPs, such as NF-κB p65, Sulfiredoxin-1(SRXN1) and Thioredoxin-interacting protein (TXNIP), were carried out using immunoblot and Real-time PCR analysis, showing that t-Cig may have a greater influence than e-Cig on tumor development and metastasis. Taken together, the finding reported here strongly support our hypothesis that electronic cigarettes are significantly less toxic compared with traditional cigarette. Highlights: Electronic cigarette is significantly less cytotoxic compared with traditional cigarette. Traditional cigarette has a greater influence than electronic cigarette on exosomal protein expression in HBE cells. Differential expressed exosomal proteins (DEEPs) in traditional cigarette group were enriched in pathways in cancer. More DEEPs related with lung cancer progression existed in traditional cigarette group than in electronic cigarette group.