Electrospun coaxial nanofibers loading with perovskite and icariin to enhance the bone scaffold-mediated osteogenesis. (December 2022)

• Record Type: Journal Article
• Authors: Zhang, Sai; Zhang, Mei; Bai, Rubing; Kong, Lingqian; Yang, Hongfang; Zhang, Anhui; Dong, Shuo; Chen, Mengyi; Ramakrishna, Seeram; Yang, Fan
• Is Part Of: Materials today chemistry. Volume 26(2022)
• Publication Date: 2022-12
• Subjects: Coaxial electrospinning -- Composite nanofibers -- Na2La2Ti3O10 nanoparticles -- Traditional Chinese medicine -- Bone tissue regeneration; Chemistry -- Periodicals; Materials -- Research -- Periodicals; Materials science -- Periodicals; Chemistry; Materials -- Research; Electronic journals; Periodicals; 660.282
• DOI: 10.1016/j.mtchem.2022.101246
• Languages: English
• ISSNs: 2468-5194

Abstract

Abstract: Electrospun nanofibers with high specific surface area and excellent extracellular matrix (ECM)-biomimetic morphology have aroused intensive interest as ideal bone tissue engineering scaffolds. Considering the inorganic/organic nanocomposite characteristics of native bone, a co-electrospinning strategy was utilized to generate innovative core-sheath nanofibers as a potential bone scaffold candidate. The core was constructed with icariin (ICA) loaded hyaluronic acid (HyA), while the sheath was made from perovskite (Na2 La2 Ti3 O10, NLT) nanoparticle loaded poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV). The as-developed PHBV/NLT-HyA/ICA nanofiber scaffold possessed stable network structure, great surface hydrophilicity (88.5 ± 2.0°), and superior mechanical performances (6.3 ± 0.6 MPa for ultimate strength and 133.8 ± 17.3 MPa for Young's modulus). Importantly, ICA was demonstrated to exhibit a sustained release profile from the coaxial nanofibers. The cumulative drug release was 74.52% after 24 days. The biological tests displayed that the PHBV/NLT-HyA/ICA nanofiber scaffold significantly promoted the viability and proliferation, and phenotypic maturation of human fetal osteoblasts (HFOBs). Moreover, the contents of alkaline phosphatase (ALP) and calcium were found to be highest in the cell-seeded PHBV/NLT-HyA/ICA nanofiber scaffold group due to a combination of NLT loading and ICA release. The present study provides an innovative strategy to construct coaxial nanofiber scaffold with PHBV/NLT as sheath and HyA/ICA as core for bone tissue engineering. Graphical abstract: Image 1 Highlights: Na2 La2 Ti3 O10 (NLT) nanoparticles were synthesized and electrospun into core-sheath nanofibers for bone tissue repair firstly. Icariin (ICA) was loaded in coaxial nanofibers to decrease burst drug release and achieve sustainable delivery. The synergistic effect of NLT, hyaluronic acid (HyA) and ICA in scaffold enhanced cell proliferation and mineralization.