SRSF1 promotes ASMC proliferation in asthma by competitively binding CCND2 with miRNA-135a. (December 2022)

• Record Type: Journal Article
• Authors: Guo, Ya-li; Chen, Zhuo-chang; Li, Nan; Tian, Cui-jie; Cheng, Dong-jun; Tang, Xue-yi; Zhang, Luo-xian; Zhang, Xiao-yu
• Is Part Of: Pulmonary pharmacology & therapeutics. Volume 77(2022)
• Publication Date: 2022-12
• Subjects: Asthma -- Airway smooth muscle cells -- SRSF1 -- miR-135a -- CCND2; ASMCs airway smooth muscle cells -- RBPs RNA-binding proteins -- SRSF1 Serine arginine-rich splicing factor 1 -- OVA ovalbumin -- qRT-PCR quantitative real-time PCR -- RIP RNA immunoprecipitation -- HE Hematoxylin-eosin -- BCA Bicinchoninic Acid -- CCND2 Cyclin D2 -- miRNAs Micro-RNAs -- mRNA messenger RNA -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- LNA locked nucleic acid; Respiratory organs -- Diseases -- Chemotherapy -- Periodicals; 615.7205
• DOI: 10.1016/j.pupt.2022.102173
• Languages: English
• ISSNs: 1094-5539

Abstract

Abstract: Background: Asthma is an inflammatory syndrome characterized by airway hyperresponsiveness, bronchial inflammation, and airway remodeling. Abnormal proliferation of airway smooth muscle cells (ASMCs) is the main pathological feature of asthma. This study investigated the function and mechanism of serine arginine-rich splicing factor 1 (SRSF1) in ASMC proliferation in asthma. Methods: SRSF1 expressions in the bronchi of ovalbumin-induced asthmatic mice and IgE-treated mouse ASMCs (mASMCs) were evaluated using quantitative real-time PCR and Western blot. The localization and expression of SRSF1 in the bronchi of asthmatic mice were assessed by immunohistochemistry. Functionally, gain- and loss-of-function assays, flow cytometry, and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assays were conducted. Mechanistically, RNA degradation assay, RNA immunoprecipitation, RNA pull-down, and dual-luciferase reporter gene assays were carried out. Results: SRSF1 was highly expressed in the bronchi of ovalbumin-induced asthma mice and IgE-treated mASMCs and was mainly located in the nucleus. Experiments on the function of SRSF1 showed that the silencing of SRSF1 induced the cell cycle of mASMC arrest and restrained mASMC proliferation. Investigations into the mechanism of SRSF1 revealed that SRSF1 and miR-135a are competitively bound to the 3′UTR region of Cyclin D2 (CCND2). SRSF1 overexpression repressed the degradation of CCND2 mRNA, and miR-135a negatively regulated CCND2 expression. Furthermore, SRSF1 knockdown inhibited ASMC proliferation in asthma mouse models by regulating the levels of miR-135a and CCND2. Conclusion: SRSF1 knockdown repressed ASMC proliferation in asthma by regulating miR-135a/CCND2 levels.