The measurement of NRF2 and TP53 in blood expects radiotherapeutic sensitivity in patients with esophageal cancer. (December 2022)

• Record Type: Journal Article
• Authors: Xu, Huiqin; Wu, Jinchang; Zhang, Lansheng; Li, yang; Gao, Liyan; Cheng, Yufeng
• Is Part Of: Molecular and cellular probes. Volume 66(2022)
• Publication Date: 2022-12
• Subjects: Esophageal cancer -- NRF2 -- TP53 -- RT-PCR -- Radiation therapy -- Radiation sensitivity; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2022.101860
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Objective: This study investigates the relationship between the mRNA expression of nuclear factor erythroid 2-related factor 2 (NRF2) and Tumor protein p53 (TP53) in circulating tumor cells (CTC) and sensitivity to radiotherapy in patients with esophageal cancer. To investigate the relationship between cytokines IL-6, CD8 +, and NRF2 during patient treatment and their predictive role for treatment. Methods: Radiosensitivity was assessed by measuring a morphological or functional change in the tumor in response to ionizing radiation. Fasting venous anticoagulated blood (EDTA anticoagulation) was drawn from patients, and the Trizol-chloroform two-step method was used for RNA extraction. Data were collected from 45 patients admitted with radiotherapy alone from January 2018 to December 2021. The expression levels of NRF2mRNA (Messenger Ribose Nucleic Acid) and TP53mRNA in CTCs were detected by reverse transcription-polymerase chain reaction (RT-PCR). Pre- and post-treatment changes in IL-6 and CD8 + were recorded. The correlation between their expression level and the clinical stage, radiotherapy sensitivity, and efficacy of patients was analyzed. Results: Twenty-six cases were sensitive to radiotherapy, and 19 were resistant, for a radiotherapy sensitivity rate of 58.8%. NRF2mRNA and TP53mRNA values increased in 19 radiotherapy-resistant patients and decreased in 26 radiotherapy-sensitive patients compared with those before radiotherapy (P = 0.001, P＜0.05). The ΔCT values of NRF2mRNA and TP53mRNA before treatment were moderately correlated with prognosis (P < 0.002). Inflammatory cytokine IL-6 was elevated in 22 of 45 patients after radiation, P = 0.04. NRF2 mRNA level was consistently elevated with CD8 + in 10 patients, P = 0.02. Conclusions: The expression of NRF2mRNA and TP53mRNA in the CTCs found in the peripheral blood of patients with esophageal squamous carcinoma was significantly associated with the sensitivity to radiotherapy. NRF2 mRNA level was consistently elevated with CD8 + and IL-6 in patients. Highlights: RT-PCR was made to explore a less invasive and patient-friendly method of predicting genetic liquid biopsies. It can potentially predict radiotherapy sensitivity before radiotherapy for patients with esophageal squamous carcinoma. Radiotherapy induces an increase in NRF2, which in turn inhibits tumor sensitivity to radiotherapy.