Fluorescent indicators for imaging membrane potential of organelles. (December 2022)

• Record Type: Journal Article
• Authors: Klier, Pavel E.Z.; Roo, Ryan; Miller, Evan W.
• Is Part Of: Current opinion in chemical biology. Volume 71(2022)
• Publication Date: 2022-12
• Subjects: Physiology -- Membrane potential -- Fluorescent dyes -- Organelles; Bioorganic chemistry -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Clinical biochemistry -- Periodicals; Biochemistry -- Periodicals; Chimie bio-organique -- Périodiques; Biologie -- Périodiques; 572.05
• DOI: 10.1016/j.cbpa.2022.102203
• Languages: English
• ISSNs: 1367-5931

Abstract

Abstract: Plasma membrane potential is a key driver of the physiology of excitable cells like neurons and cardiomyocytes. Voltage-sensitive fluorescent indicators offer a powerful complement to traditional electrode-based approaches to measuring and monitoring membrane potential. Intracellular organelles can also generate membrane potential, yet the electrode- and fluorescent indicator-based approaches used for plasma membrane potential imaging are difficult to implement on intact organelles in their native environment. Here, we survey recent advances in developing and deploying voltage-sensitive fluorescent indicators to interrogate organelle membrane potential in intact cells.