Caffeine intoxication: Behavioral and electrocorticographic patterns in Wistar rats. (December 2022)

• Record Type: Journal Article
• Authors: Azevedo, Julianne Elba Cunha; da Silva, Alex Luiz Menezes; Vieira, Luana Rodrigues; Nascimento, Chirlene Pinheiro; Pereira, Rafaela Garcia; Rodrigues, Sofia de França; Hamoy, Akira Otake; Mello, Vanessa Joia de; Araújo, Daniella Bastos de; Barbas, Luis André Luz; Lopez, Maria Elena Crespo; Lopes, Dielly Catrina Favacho; Hamoy, Moisés
• Is Part Of: Food and chemical toxicology. Volume 170(2022)
• Publication Date: 2022-12
• Subjects: Caffeine -- Seizures -- Electrocorticography -- Intoxication -- Toxicology; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2022.113452
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Caffeine is a psychoactive substance used worldwide. The present study analyzes the seizure-related behavior and electrocorticographic (ECoG) patterns observed in rats following of a toxic dose of caffeine (150 mg/kg; intraperitoneal). Sixty-three rats were divided into three experiments: 1-Behavior's Description associated with caffeine-induced convulsion; 2- Comparison of the electrocorticographic patterns induced by caffeine and pentylenetetrazole, and 3- Assessment of the electrocorticographic response to antiepileptic drugs (diazepam, phenytoin, and phenobarbital). The behavioral analysis demonstrated tonic-clonic seizures with a loss of postural reflex and a latency of 365.8 s after the caffeine's administration. Caffeine-induced changes in the ECoG were consistent with the development of seizures with rapid evolution and burst potential consistent with the behavioral patterns observed during the caffeine-induced seizure. The ECoG of the brainwaves varied significantly between the seizures caused by caffeine and pentylenetetrazole. The predominant brain forces observed during the seizures were beta-band oscillations. The caffeine-induced seizures were resistant to attempted control with phenytoin and phenobarbital, but responded well to diazepam, which is consistent with a study of Pilocarpine, which showed that diazepam has anticonvulsant effects. These findings are important for the development of effective treatments for caffeine intoxication, in particular for individuals with a low seizure threshold. Highlights: Toxic dose of caffeine-induced convulsion. The predominant brain forces observed during the seizures were beta-band oscillations. The caffeine-induced seizures responded well to diazepam.