The influence of perinatal maternal exposure to dibutyl phthalate on glucolipid metabolism in adult female offspring. Issue 6 (November 2022)

• Record Type: Journal Article
• Authors: Zhou, Kunyan; Cheng, Ran; Zhu, Mei; Yang, Meina; Shen, Xiaoyang; Luo, Xiaoyan; Ma, Li; Xu, Liangzhi; Zhang, Jing
• Is Part Of: Obesity research & clinical practice. Volume 16:Issue 6(2022)
• Page Start: 500
• Page End: 506
• Publication Date: 2022-11
• Subjects: DBP dibutyl phthalate -- PND post-natal day -- Tch total cholesterol -- TG triglyceride -- FG fasting glucose -- PRL prolactin -- FINS fasting insulin -- ADP adiponectin -- ELISA enzyme-linked immunosorbent assay -- HOMA-IR homeostatic model assessment of estimated insulin resistance -- InsR insulin receptor -- IRS-1 insulin receptor substrate-1 -- PI3K phosphatidylinositol 3-hydroxy kinase -- p-AKT phosphorylated protein kinase B -- GLUT4 glucose transporter 4 -- PTEN phosphatase and tensin homology deleted on chromosome 10 -- GRP30 G protein coupled estrogen receptor 30 -- BCA bicinchoninic acid -- PVDF polyvinylidene fluoride -- SD standard deviation -- ANOVA analysis of variance -- PPAR peroxisome proliferator-activated receptor; Dibutyl phthalate -- Obesity -- Visceral fat -- Glucolipid metabolism -- PI3K -- PTEN; Obesity -- Research -- Periodicals; Obesity -- Treatment -- Periodicals; Obesity -- Periodicals; Obésité -- Recherche -- Périodiques; Obésité -- Traitement -- Périodiques; Obesity -- Research; Obesity -- Treatment; Electronic journals; Periodicals; 616.398
• DOI: 10.1016/j.orcp.2022.10.007
• Languages: English
• ISSNs: 1871-403X

Abstract

Abstract: Background: Maternal exposure to dibutyl phthalate (DBP) may result in obesity in female offspring. However, the underlying mechanisms remain elusive. Materials and methods: Sprague-Dawley rats were intraperitoneally injected with different doses of DBP and corn oil from gestational day 7 until the end of lactation. The weights, visceral fat percentage, serum lipid, insulin and glucose, protein levels of PI3K signal pathway in muscle were detected in F1 female offspring. Results: Although the birth weight of F1 female offspring was not different among groups, the weights were heavier in DBP groups from postnatal day 7 to adult (P < 0.001). The visceral adipose percentage in adult female offspring was increased by perinatal exposure to DBP (P < 0.001). Decreased serum level of triglyceride (P = 0.001) in F1 female offspring was found in DBP group as compared to control, especially in medium and high DBP. However, none difference was found for fasting glucose, prolactin, HOMA-IR, fasting insulin, total cholesterol, adiponectin. Different protein levels of GPR30 were observed in muscle of female offspring among four groups (P = 0.016). The protein level of AKT seemed higher in DBP group but without statistical significance (P = 0.05). None difference was observed for the protein levels of PI3K, p-AKT, pAKT/AKT, PTEN, GLUT4, InsR, IRS. Conclusion: Maternal perinatal exposure to DBP might induce obesity and accumulation of visceral adipose tissue for the adult female offspring. Serum glucolipid and local signal transduction of PTEN/PI3K/AKT pathway in muscle were not adversely affected by perinatal exposure to DBP for adult female offspring.