Genomics‐Driven Discovery of NO‐Donating Diazeniumdiolate Siderophores in Diverse Plant‐Associated Bacteria. (5th August 2019)

• Record Type: Journal Article
• Authors: Hermenau, Ron; Mehl, Jule L.; Ishida, Keishi; Dose, Benjamin; Pidot, Sacha J.; Stinear, Timothy P.; Hertweck, Christian
• Is Part Of: Angewandte Chemie. Volume 131:Number 37(2019)
• Page Start: 13158
• Page End: 13163
• Publication Date: 2019-08-05
• Subjects: Burkholderia -- Diazeniumdiolat -- Nicht-ribosomale Peptide -- Siderophore -- Stickstoffmonoxid; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201906326
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Siderophores are key players in bacteria–host interactions, with the main function to provide soluble iron for their producers. Gramibactin from rhizosphere bacteria expands siderophore function and diversity as it delivers iron to the host plant and features an unusual diazeniumdiolate moiety for iron chelation. By mutational analysis of the grb gene cluster, we identified genes (grbD and grbE) necessary for diazeniumdiolate formation. Genome mining using a GrbD‐based network revealed a broad range of orthologous gene clusters in mainly plant‐associated Burkholderia/Paraburkholderia species. Two new types of diazeniumdiolate siderophores, megapolibactins and plantaribactin were fully characterized. In vitro assays and in vivo monitoring experiments revealed that the iron chelators also liberate nitric oxide (NO) in plant roots. This finding is important since NO donors are considered as biofertilizers that maintain iron homeostasis and increase overall plant fitness. Abstract : Wurzelbehandlung : Sehr ungewöhnliche Siderophore wurden in vielfältigen pflanzenassoziierten Bakterien entdeckt. Erste Einblicke in die genetischen Grundlagen ihrer Biosynthese ermöglichten das gezielte Genom‐Mining nach nicht‐ribosomalen Peptiden mit Diazeniumdiolat ( N ‐Nitrosohydroxylamin)‐Einheiten zur Eisenchelatisierung. Die seltenen N ‐Nitroso‐Gruppen setzen zudem NO, ein wichtiges Pflanzenhormon, in Pflanzenwurzeln frei.