Genomics‐Driven Discovery of NO‐Donating Diazeniumdiolate Siderophores in Diverse Plant‐Associated Bacteria. Issue 37 (5th August 2019)

• Record Type: Journal Article
• Authors: Hermenau, Ron; Mehl, Jule L.; Ishida, Keishi; Dose, Benjamin; Pidot, Sacha J.; Stinear, Timothy P.; Hertweck, Christian
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 37(2019)
• Page Start: 13024
• Page End: 13029
• Publication Date: 2019-08-05
• Subjects: Burkholderia -- diazeniumdiolate -- nitric oxide -- non-ribosomal peptide -- siderophores; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201906326
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Siderophores are key players in bacteria–host interactions, with the main function to provide soluble iron for their producers. Gramibactin from rhizosphere bacteria expands siderophore function and diversity as it delivers iron to the host plant and features an unusual diazeniumdiolate moiety for iron chelation. By mutational analysis of the grb gene cluster, we identified genes (grbD and grbE) necessary for diazeniumdiolate formation. Genome mining using a GrbD‐based network revealed a broad range of orthologous gene clusters in mainly plant‐associated Burkholderia/Paraburkholderia species. Two new types of diazeniumdiolate siderophores, megapolibactins and plantaribactin were fully characterized. In vitro assays and in vivo monitoring experiments revealed that the iron chelators also liberate nitric oxide (NO) in plant roots. This finding is important since NO donors are considered as biofertilizers that maintain iron homeostasis and increase overall plant fitness. Abstract : NO News : Highly unusual siderophores were discovered in a broad range of plant‐associated bacteria. First insights into the genetic basis of their biosynthesis enabled targeted genome mining for non‐ribosomal peptides featuring diazeniumdiolate ( N ‐nitroso‐hydroxylamine) moieties for iron chelation. Furthermore, the rare N ‐nitroso groups liberate nitric oxide (NO), an important plant hormone, in plant roots.