DNP‐Supported Solid‐State NMR Spectroscopy of Proteins Inside Mammalian Cells. Issue 37 (19th July 2019)

• Record Type: Journal Article
• Authors: Narasimhan, Siddarth; Scherpe, Stephan; Lucini Paioni, Alessandra; van der Zwan, Johan; Folkers, Gert E.; Ovaa, Huib; Baldus, Marc
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 37(2019)
• Page Start: 12969
• Page End: 12973
• Publication Date: 2019-07-19
• Subjects: dynamic nuclear polarization -- in-cell NMR -- protein–protein interactions -- solid-state NMR -- ubiquitination; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201903246
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Elucidating at atomic level how proteins interact and are chemically modified in cells represents a leading frontier in structural biology. We have developed a tailored solid‐state NMR spectroscopic approach that allows studying protein structure inside human cells at atomic level under high‐sensitivity dynamic nuclear polarization (DNP) conditions. We demonstrate the method using ubiquitin (Ub), which is critically involved in cellular functioning. Our results pave the way for structural studies of larger proteins or protein complexes inside human cells, which have remained elusive to in‐cell solution‐state NMR spectroscopy due to molecular size limitations. Abstract : A solid‐state NMR spectroscopy approach is introduced to study proteins inside eukaryotic cells at endogenous expression levels under high‐sensitivity dynamic nuclear polarization (DNP) conditions. This approach was demonstrated using ubiquitin and the amino acids that could be identified are highlighted in red. These results pave the way for structural studies of biomolecules and their complexes inside human cells at atomic resolution.