Down-regulation of miR-140-3p is a cause of the interlukin-13-induced up-regulation of RhoA protein in bronchial smooth muscle cells. (31st December 2022)

• Record Type: Journal Article
• Authors: Chiba, Yoshihiko; Ando, Yusuke; Kato, Yasuna; Hanazaki, Motohiko; Sakai, Hiroyasu
• Is Part Of: Small GTPases. Volume 13:Number 1(2022)
• Page Start: 1
• Page End: 6
• Publication Date: 2022-12-31
• Subjects: Microrna (miRNA) -- miR-140-3p -- RhoA -- asthma -- bronchial smooth muscle hyperresponsiveness; Guanosine triphosphatase -- Periodicals; Guanosine triphosphatase; Periodicals; 572.793
• DOI: 10.1080/21541248.2021.1872318
• Languages: English
• ISSNs: 2154-1256

Abstract

ABSTRACT: The current study aimed to determine the role of a microRNA (miRNA), miR-140-3p, in the control of RhoA expression in bronchial smooth muscle cells (BSMCs). In cultured human BSMCs, incubation with interleukin-13 (IL-13) caused an up-regulation of RhoA protein concurrently with a down-regulation of miR-140-3p. Transfection of the cells with a miR-140-3p inhibitor caused an increase in basal RhoA protein level. Although a mimic of miR-140-3p had little effect on the basal RhoA level, its treatment inhibited the IL-13-induced up-regulation of RhoA. These findings suggest that RhoA expression is negatively regulated by miR-140-3p, and that the negative regulation is inhibited by IL-13 to cause an up-regulation of RhoA protein in BSMCs.