Luminescence detection of peptide:N-glycanase activity using engineered split inteins. Issue 95 (14th November 2022)
- Record Type:
- Journal Article
- Title:
- Luminescence detection of peptide:N-glycanase activity using engineered split inteins. Issue 95 (14th November 2022)
- Main Title:
- Luminescence detection of peptide:N-glycanase activity using engineered split inteins
- Authors:
- Takahashi, Tsuyoshi
Uchibayashi, Tatsuya
Ishii, Nozomi
Matsuo, Ichiro
Yoshida, Yukiko
Suzuki, Tadashi - Abstract:
- Abstract : A convenient method to detect peptide: N -glycanase activity was developed using engineered split inteins in which the N-intein sequences contain N, N ′-diacetylchitobiose. Abstract : A split intein-based method has been developed to detect peptide: N -glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N, N ′-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.
- Is Part Of:
- Chemical communications. Volume 58:Issue 95(2022)
- Journal:
- Chemical communications
- Issue:
- Volume 58:Issue 95(2022)
- Issue Display:
- Volume 58, Issue 95 (2022)
- Year:
- 2022
- Volume:
- 58
- Issue:
- 95
- Issue Sort Value:
- 2022-0058-0095-0000
- Page Start:
- 13282
- Page End:
- 13285
- Publication Date:
- 2022-11-14
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/d2cc04865e ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 24428.xml