Compromised regulation of the collecting duct ENaC activity in mice lacking AT1a receptor. Issue 9 (25th March 2018)

• Record Type: Journal Article
• Authors: Mamenko, Mykola; Zaika, Oleg; Tomilin, Viktor; Jensen, V.Behrana; Pochynyuk, Oleh
• Is Part Of: Journal of cellular physiology. Volume 233:Issue 9(2018:Sep.)
• Page Start: 7217
• Page End: 7225
• Publication Date: 2018-03-25
• Subjects: aldosterone -- distal renal tubule -- Mas -- mineralocorticoid receptors -- Na+ reabsorption; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.26552
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : ENaC‐mediated sodium reabsorption in the collecting duct (CD) is a critical determinant of urinary sodium excretion. Existing evidence suggest direct stimulatory actions of Angiotensin II (Ang II) on ENaC in the CD, independently of the aldosterone‐mineralocorticoid receptor (MR) signaling. Deletion of the major renal AT1 receptor isoform, AT1a R, decreases blood pressure and reduces ENaC abundance despite elevated aldosterone levels. The mechanism of this insufficient compensation is not known. Here, we used patch clamp electrophysiology in freshly isolated split‐opened CDs to investigate how AT1a R dysfunction compromises functional ENaC activity and its regulation by dietary salt intake. Ang II had no effect on ENaC activity in CDs from AT1a R −/− mice suggesting no complementary contribution of AT2 receptors. We next found that AT1a R deficient mice had lower ENaC activity when fed with low (<0.01% Na + ) and regular (0.32% Na + ) but not with high (∼2% Na + ) salt diet, when compared to the respective values obtained in Wild type (WT) animals. Inhibition of AT1 R with losartan in wild‐type animals reproduces the effects of genetic ablation of AT1a R on ENaC activity arguing against contribution of developmental factors. Interestingly, manipulation with aldosterone‐MR signaling via deoxycosterone acetate (DOCA) and spironolactone had much reduced influence on ENaC activity upon AT1a R deletion. Consistently, AT1a R −/− mice have a markedly diminished MR abundance in cytosol. Overall, we conclude that AT1a R deficiency elicits a complex inhibitory effect on ENaC activity by attenuating ENaC Po and precluding adequate compensation via aldosterone cascade due to decreased MR availability. Abstract : We used patch clamp electrophysiology in freshly isolated split‐opened CDs to investigate how AT1aR dysfunction compromises functional ENaC activity and its regulation by dietary salt intake. We found that AT1aR deficient mice had lower ENaC activity when fed with low (<0.01% Na+) and regular (0.32% Na+) but not with high (∼2% Na+) salt diet, when compared to the respective values obtained in Wild type (WT) animals. AT1aR −/− mice also have a markedly diminished MR abundance in cytosol. Overall, we conclude that AT1aR deficiency elicits a complex inhibitory effect on ENaC activity by attenuating ENaC Po and precluding adequate compensation via aldosterone cascade due to decreased MR availability.