Precise epigenomic editing with a SunTag-based modular epigenetic toolkit. Issue 13 (9th December 2022)

• Record Type: Journal Article
• Authors: Guhathakurta, Subhrangshu; Adams, Levi; Jeong, Inhye; Sivakumar, Anishaa; Cha, Mingyu; Bernardo Fiadeiro, Mariana; Hu, Haiyan Nancy; Kim, Yoon-Seong
• Is Part Of: Epigenetics. Volume 17:Issue 13(2022)
• Page Start: 2075
• Page End: 2081
• Publication Date: 2022-12-09
• Subjects: Epigenetics -- histone post-translational modifications -- epigenome editing -- CRISPR -- epigenetic writers; Epigenesis -- Periodicals; Epigenetica; 572.86505
• DOI: 10.1080/15592294.2022.2106646
• Languages: English
• ISSNs: 1559-2294

Abstract

ABSTRACT: Epigenetic regulation is a crucial factor controlling gene expression. Here, we report our CRISPR/dCas9-based modular epigenetic toolkit that enables gene-specific modulation of epigenetic architecture. By modifying the SunTag framework of dCas9 tagged with five GCN4 moieties, each epigenetic writer is bound to scFv and target-specific sgRNA, and this system is able to modify multiple epigenetic marks in a target-specific manner. We successfully demonstrated that this system is efficient in modifying individual histone post-translational modifications. We display its utility as a tool to understand the contributions of specific histone marks on gene expression by screening a large promoter region and identifying differential outcomes with high base-pair resolution. This epigenetic toolkit can be easily altered with a large variety of epigenetic effectors and is a useful tool for researchers to use in understanding gene-specific epigenetic changes and their relation to gene expression.