Determination of γ-D-glutamyl-meso-diaminopimelic acid in rumen fluid of dairy cows by pre-column chiral derivatization-HPLC. Issue 6 (18th November 2022)

• Record Type: Journal Article
• Authors: Wang, Yan; Gao, Qianyun; Cheng, Xiaoye; Chang, Guangjun; Roy, Animesh Chandra; Shen, Xiangzhen
• Is Part Of: Animal biotechnology. Volume 33:Issue 6(2022)
• Page Start: 1109
• Page End: 1117
• Publication Date: 2022-11-18
• Subjects: Chiral derivatization -- dairy cow -- high-concentrate diet -- high-performance liquid chromatography -- iE-DAP; Animal biotechnology -- Periodicals; Animals, Domestic -- genetics -- Periodicals; Animals, Genetically Modified -- Periodicals; 660.6
• DOI: 10.1080/10495398.2020.1870484
• Languages: English
• ISSNs: 1049-5398

Abstract

Abstract: High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ -D-glutamyl- meso -diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is the minimum PGN structure capable of activating inflammatory signaling pathways. This research paper aimed to determine the iE-DAP concentration and investigate the effects of an HC diet on the concentration of iE-DAP in the rumen fluid of dairy cows. However, there are limited studies on the determination of iE-DAP concentration. Hence, we established a high-performance liquid chromatography (HPLC) method combined with pre-column chiral derivatization to detect the concentration of iE-DAP in rumen fluid. Moreover, we conducted an animal experiment that included 12 lactating Holstein cows, which were randomly divided into a low-concentrate (LC) group and an HC group. The results showed that the linear range of iE-DAP was 5–500 µg/mL and that the intra- and inter-day RSDs were lower than 7%. Meanwhile, this method was successfully applied to the analysis of iE-DAP in rumen fluid, and the results revealed that long-term feeding with an HC diet elevated the concentration of iE-DAP in rumen fluid of dairy cows.