MiR-421 regulates goat intramuscular preadipocytes differentiation via targeting FGF13. Issue 6 (18th November 2022)

• Record Type: Journal Article
• Authors: Du, Yu; Ma, Jieqiong; Wang, Yong; Zhu, Jiangjiang; Li, Yanyan; Meng, Qingyong; Lin, Yaqiu
• Is Part Of: Animal biotechnology. Volume 33:Issue 6(2022)
• Page Start: 1333
• Page End: 1343
• Publication Date: 2022-11-18
• Subjects: miR-421 -- FGF13 -- goat intramuscular preadipocytes -- differentiation; Animal biotechnology -- Periodicals; Animals, Domestic -- genetics -- Periodicals; Animals, Genetically Modified -- Periodicals; 660.6
• DOI: 10.1080/10495398.2021.1898414
• Languages: English
• ISSNs: 1049-5398

Abstract

Abstract: As a member of the MicroRNA s (miRNAs) family, miR-421 has been widely studied in regulating the proliferation and apoptosis of cancer cells a. However, there are still no reports on miR-421 in regulating adipocyte differentiation and its related mechanisms. Accordingly, this study aimed to investigate the potential involvement of miR-421 in goat intramuscular preadipocytes (P_IMA). The expression level of miR-421 was measured via quantitative real-time PCR during goat P_IMA differentiation. And the effects of miR-421 on goat P_IMA differentiation were studied by liposome transfection, Oil red O staining and qRT-PCR. Furthermore, the miR-421 target was searched and the underlying mechanism was clarified by luciferase reporter assay and rescue experiment. Our results showed that inhibition of miR-421 could accumulation of lipid droplets by upregulation the expression level of AP 2, LPL, C/EBPα and SREBP1 . Further studies showed that fibroblast growth factor 13 ( FGF13 ) was the direct target of miR-421. Knocking down of FGF13 expression could inhibit lipid droplet formation and down-regulated the expression of key adipogenic regulatory genes. In addition, the rescue experiment revealed that FGF13 is involved in miR-421-induced differentiation of goat P_IMA as a key factor. Overall, these findings indicate that miR-421 is a negative regulator in the progression of differentiation of goat P_IMA by inhibiting the expression of FGF13 .